FN Archimer Export Format
PT J
TI Lipid-free tuna muscle samples are suitable for total mercury analysis
BT 
AF Médieu, Anais
   Sardenne, Fany
   Lorrain, Anne
   Bodin, Nathalie
   Pazart, Chloé
   Le Delliou, Herve
   Point, David
AS 1:5;2:7;3:6;4:2,3;5:5;6:1;7:4;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-PFOM-LARN;7:;
C1 Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
   Research Institute for Sustainable Development (IRD), Victoria, Mahé, Seychelles
   Sustainable Ocean Seychelles (SOS), BeauBelle, Mahé, Seychelles
   Observatoire Midi-Pyrénées, GET, UMR CNRS 5563/IRD 234, Université Paul Sabatier Toulouse 3, Toulouse, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
C2 IFREMER, FRANCE
   IRD, SEYCHELLES
   SUSTAINABLE OCEAN SEYCHELLES (SOS), SEYCHELLES
   OBSERV MIDI PYRENEES, FRANCE
   UBO, FRANCE
   IRD, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-RBE-PFOM-LARN
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
IF 3.737
TC 2
UR https://archimer.ifremer.fr/doc/00697/80930/84804.pdf
LA English
DT Article
DE ;Methylmercury;Fat content;Delipidation;Yellowfin;Bigeye;Skipjack
AB Tropical tunas are largely consumed worldwide, providing major nutritional benefits to humans, but also representing the main exposure to methylmercury, a potent neurotoxin that biomagnifies along food webs. The combination of ecological tracers (nitrogen and carbon stable isotopes, δ15N and δ13C) to mercury concentrations in tunas is scarce yet crucial to better characterise the influence of tuna foraging ecology on mercury exposure and bioaccumulation. Given the difficulties to get modern and historical tuna samples, analyses have to be done on available and unique samples. However, δ13C values are often analysed on lipid-free samples to avoid bias related to lipid content. While lipid extraction with non-polar solvents is known to have no effect on δ15N values, its impact on mercury concentrations is still unclear. We used white muscle tissues of three tropical tuna species to evaluate the efficiency and repeatability of different lipid extraction protocols commonly used in δ13C and δ15N analysis. Dichloromethane was more efficient than cyclohexane in extracting lipids in tuna muscle, while the automated method appeared more efficient but as repeatable as the manual method. Lipid extraction with dichloromethane had no effect on mercury concentrations. This may result from i) the affinity of methylmercury to proteins in tuna flesh, ii) the low lipid content in tropical tuna muscle samples, and iii) the non-polar nature of dichloromethane. Our study suggests that lipid-free samples, usually prepared for tropical tuna foraging ecology research, can be used equivalently to bulk samples to document in parallel mercury concentrations at a global scale. 
PY 2021
PD JUN
SO Marine Environmental Research
SN 0141-1136
PU Elsevier BV
VL 169
UT 000675833400001
DI 10.1016/j.marenvres.2021.105385
ID 80930
ER

EF