TY - JOUR T1 - Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase A1 - Hajj,Mirna A1 - Langendijk-Genevaux,Petra A1 - Batista,Manon A1 - Quentin,Yves A1 - Laurent,Sebastien A1 - Capeyrou,Régine A1 - Abdel-Razzak,Ziad A1 - Flament,Didier A1 - Chamieh,Hala A1 - Fichant,Gwennaele A1 - Clouet-D’orval,Béatrice A1 - Bouvier,Marie AD - Laboratoire de Microbiologie et Génétique Moléculaires, UMR5100, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, Université Paul Sabatier, F-31062 Toulouse, France AD - Laboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and Its Application, EDST, Lebanese University, Tripoli 1300, Lebanon AD - Ifremer, Univ Brest, CNRS, UMR 6197 Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France AD - Laboratoire de Biologie Moléculaire, Cellulaire et du Développement, UMR5077, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, Université Paul Sabatier, F-31062 Toulouse, France UR - https://archimer.ifremer.fr/doc/00702/81418/ DO - 10.3390/biom11070950 KW - SF2 helicases KW - aLhr2 helicases KW - archaea KW - RNA metabolism KW - DNA repair KW - Thermococcales N2 - Helicase proteins are known to use the energy of ATP to unwind nucleic acids and to remodel protein-nucleic acid complexes. They are involved in almost every aspect of DNA and RNA metabolisms and participate in numerous repair mechanisms that maintain cellular integrity. The archaeal Lhr-type proteins are SF2 helicases that are mostly uncharacterized. They have been proposed to be DNA helicases that act in DNA recombination and repair processes in Sulfolobales and Methanothermobacter. In Thermococcales, a protein annotated as an Lhr2 protein was found in the network of proteins involved in RNA metabolism. To investigate this, we performed in-depth phylogenomic analyses to report the classification and taxonomic distribution of Lhr-type proteins in Archaea, and to better understand their relationship with bacterial Lhr. Furthermore, with the goal of envisioning the role(s) of aLhr2 in Thermococcales cells, we deciphered the enzymatic activities of aLhr2 from Thermococcus barophilus (Tbar). We showed that Tbar-aLhr2 is a DNA/RNA helicase with a significant annealing activity that is involved in processes dependent on DNA and RNA transactions Y1 - 2021/07 PB - MDPI AG JF - Biomolecules SN - 2218-273X VL - 11 IS - 7 ID - 81418 ER -