FN Archimer Export Format
PT J
TI Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase
BT 
AF Hajj, Mirna
   Langendijk-Genevaux, Petra
   Batista, Manon
   Quentin, Yves
   Laurent, Sebastien
   Capeyrou, Régine
   Abdel-Razzak, Ziad
   Flament, Didier
   Chamieh, Hala
   Fichant, Gwennaele
   Clouet-d’Orval, Béatrice
   Bouvier, Marie
AS 1:1,2;2:1;3:1;4:1;5:3;6:4;7:2;8:3;9:2;10:1;11:1,4;12:1,4;
FF 1:;2:;3:;4:;5:PDG-REM-EEP-LMEE;6:;7:;8:PDG-REM-EEP-LMEE;9:;10:;11:;12:;
C1 Laboratoire de Microbiologie et Génétique Moléculaires, UMR5100, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, Université Paul Sabatier, F-31062 Toulouse, France
   Laboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and Its Application, EDST, Lebanese University, Tripoli 1300, Lebanon
   Ifremer, Univ Brest, CNRS, UMR 6197 Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
   Laboratoire de Biologie Moléculaire, Cellulaire et du Développement, UMR5077, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, Université Paul Sabatier, F-31062 Toulouse, France
C2 UNIV TOULOUSE, FRANCE
   UNIV LEBANESE, LEBANON
   IFREMER, FRANCE
   UNIV TOULOUSE, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 6.064
TC 4
UR https://archimer.ifremer.fr/doc/00702/81418/85807.pdf
   https://archimer.ifremer.fr/doc/00702/81418/85808.zip
LA English
DT Article
DE ;SF2 helicases;aLhr2 helicases;archaea;RNA metabolism;DNA repair;Thermococcales
AB Helicase proteins are known to use the energy of ATP to unwind nucleic acids and to remodel protein-nucleic acid complexes. They are involved in almost every aspect of DNA and RNA metabolisms and participate in numerous repair mechanisms that maintain cellular integrity. The archaeal Lhr-type proteins are SF2 helicases that are mostly uncharacterized. They have been proposed to be DNA helicases that act in DNA recombination and repair processes in Sulfolobales and Methanothermobacter. In Thermococcales, a protein annotated as an Lhr2 protein was found in the network of proteins involved in RNA metabolism. To investigate this, we performed in-depth phylogenomic analyses to report the classification and taxonomic distribution of Lhr-type proteins in Archaea, and to better understand their relationship with bacterial Lhr. Furthermore, with the goal of envisioning the role(s) of aLhr2 in Thermococcales cells, we deciphered the enzymatic activities of aLhr2 from Thermococcus barophilus (Tbar). We showed that Tbar-aLhr2 is a DNA/RNA helicase with a significant annealing activity that is involved in processes dependent on DNA and RNA transactions 
PY 2021
PD JUN
SO Biomolecules
SN 2218-273X
PU MDPI AG
VL 11
IS 7
UT 000676681800001
DI 10.3390/biom11070950
ID 81418
ER

EF