FN Archimer Export Format PT J TI Prevalence and polymorphism of a mussel transmissible cancer in Europe BT AF Hammel, Maurine Simon, Alexis Arbiol, Christine Villalba, Antonio Burioli, Erika AV Pépin, Jean-Francois Lamy, Jean-Baptiste Benabdelmouna, Abdellah Bernard, Ismael Houssin, Maryline Charrière, Guillaume DESTOUMIEUX GARZON, Delphine Welch, John J. Metzger, Michael J Bierne, Nicolas AS 1:1,2;2:1;3:1;4:3,4,5;5:2,6;6:7;7:8;8:8;9:9;10:6;11:2;12:2;13:10;14:11;15:1; FF 1:;2:;3:;4:;5:;6:PDG-ODE-LITTORAL-LERPC;7:PDG-RBE-SGMM-LGPMM;8:PDG-RBE-SGMM-LGPMM;9:;10:;11:;12:;13:;14:;15:; C1 ISEM, Univ Montpellier, CNRS, EPHE, IRD Montpellier,France IHPE, Univ Montpellier, CNRS, Ifremer, Univ Perpignan Via Domitia, France Centro de Investigacións Mariñas Consellería do Mar, Xunta de Galicia Vilanova de Arousa ,Spain Departamento de Ciencias de la Vida Universidad de Alcalá Alcalá de Henares, Spain Research Centre for Experimental Marine Biology and Biotechnology (PIE) University of the Basque Country (UPV/EHU) Plentzia, Basque Country, Spain LABÉO, Caen, France Laboratoire Environnement ressources des Pertuis Charentais IFREMER La Tremblade ,France Santé, Génétique, Microbiologie des Mollusques, IFREMER La Tremblade, France Eurêka Mer, Lézardrieux, France Department of Genetics,University of Cambridge Downing Street, Cambridge, UK Pacific Northwest Research Institute, Seattle, USA C2 UNIV MONTPELLIER, FRANCE UNIV PERPIGNAN, FRANCE CIMA, SPAIN UNIV ALCALA DE HENARES, SPAIN UNIV PAIS VASCO EHU, SPAIN LABEO, FRANCE IFREMER, FRANCE IFREMER, FRANCE EUREKA MER, FRANCE UNIV CAMBRIDGE, UK PNRI, USA SI LA TREMBLADE SE PDG-ODE-LITTORAL-LERPC PDG-RBE-SGMM-LGPMM UM IHPE IN WOS Ifremer UPR WOS Cotutelle UMR copubli-france copubli-europe copubli-univ-france copubli-int-hors-europe IF 4.9 TC 18 UR https://archimer.ifremer.fr/doc/00703/81540/85981.pdf LA English DT Article DE ;disseminated neoplasia;genetic chimerism;genetic polymorphism;Mytilus sp;mussels;transmissible cancer AB Transmissible cancers are parasitic malignant cell lineages that acquired the ability to infect new hosts from the same species, or sometimes related species. First described in dogs and Tasmanian devils, transmissible cancers were later discovered in some marine bivalves affected by a leukemia-like disease. In Mytilus mussels, two lineages of Bivalve Transmissible Neoplasia (BTN) have been described to date (MtrBTN1 and MtrBTN2), both emerged in a M. trossulus founder individual. Here, we performed an extensive screening of genetic chimerism, a hallmark of transmissible cancer, by genotyping 106 SNPs of 5907 European Mytilus mussels. The genetic analysis allowed us to simultaneously obtain the genotype of hosts - M. edulis, M. galloprovincialis or hybrids - and the genotype of tumors of heavily infected individuals. In addition, a subset of 222 individuals were systematically genotyped and analysed by histology in order to screen for possible non-transmissible cancers. We detected MtrBTN2 at low prevalence in M.edulis, and also in M. galloprovincialis and hybrids although at a much lower prevalence. No MtrBTN1 or new BTN were found, but 8 individuals with non-transmissible neoplasia were observed at a single polluted site on the same sampling date. We observed a diversity of MtrBTN2 genotypes that appeared more introgressed or more ancestral than MtrBTN1 and reference healthy M. trossulus individuals. The observed polymorphism is most likely due to somatic null alleles caused by structural variations or point mutations in primer-binding sites leading to enhanced detection of the host alleles. Despite low prevalence, two sublineages divergent by 10% fixed somatic null alleles and one non-synonymous mtCOI substitution, are co-spreading in the same geographic area, suggesting a complex diversification of MtrBTN2 since its emergence and host species shift. PY 2022 PD FEB SO Molecular Ecology SN 0962-1083 PU Wiley VL 31 IS 3 UT 000674151700001 BP 736 EP 751 DI 10.1111/mec.16052 ID 81540 ER EF