FN Archimer Export Format
PT J
TI A phylogeny-aware approach reveals unexpected venom components in divergent lineages of cone snails
BT 
AF Fedosov, Alexander
   Zaharias, Paul
   Puillandre, Nicolas
AS 1:1,2;2:2,3;3:2;
FF 1:;2:;3:;
C1 A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russian Federation
   Institut Systématique Evolution Biodiversité (ISYEB), Muséum National d'Histoire Naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, 57 rue Cuvier, CP 26, 75005 Paris, France
   Department of Computer Science, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA
C2 RUSSIAN ACAD SCI, RUSSIAN
   MNHN, FRANCE
   UNIV ILLINOIS, USA
IF 5.531
TC 4
UR https://archimer.ifremer.fr/doc/00705/81668/86163.pdf
   https://archimer.ifremer.fr/doc/00705/81668/86164.zip
LA English
DT Article
CR KANACONO
   KAVIENG
BO Alis
DE ;conotoxins;venom evolution;phylogeny;Conidae;Conasprella;Pygmaeconus
AB Marine gastropods of the genus Conus are renowned for their remarkable diversity and deadly venoms. While Conus venoms are increasingly well studied for their biomedical applications, we know surprisingly little about venom composition in other lineages of Conidae. We performed comprehensive venom transcriptomic profiling for Conasprella coriolisi and Pygmaeconus traillii, first time for both respective genera. We complemented reference-based transcriptome annotation by a de novo toxin prediction guided by phylogeny, which involved transcriptomic data on two additional ‘divergent’ cone snail lineages, Profundiconus, and Californiconus. We identified toxin clusters (SSCs) shared among all or some of the four analysed genera based on the identity of the signal region—a molecular tag present in toxins. In total, 116 and 98 putative toxins represent 29 and 28 toxin gene superfamilies in Conasprella and Pygmaeconus, respectively; about quarter of these only found by semi-manual annotation of the SSCs. Two rare gene superfamilies, originally identified from fish-hunting cone snails, were detected outside Conus rather unexpectedly, so we further investigated their distribution across Conidae radiation. We demonstrate that both these, in fact, are ubiquitous in Conidae, sometimes with extremely high expression. Our findings demonstrate how a phylogeny-aware approach circumvents methodological caveats of similarity-based transcriptome annotation. 
PY 2021
PD JUN
SO Proceedings Of The Royal Society B-biological Sciences
SN 0962-8452
PU The Royal Society
VL 288
IS 1954
UT 000671879200010
DI 10.1098/rspb.2021.1017
ID 81668
ER

EF