FN Archimer Export Format
PT J
TI Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
BT 
AF Slobodkina, Galina
   ALLIOUX, Maxime
   Merkel, Alexander
   CAMBON BONAVITA, Marie-Anne
   ALAIN, Karine
   JEBBAR, Mohamed
   Slobodkin, Alexander
AS 1:1;2:4;3:1;4:2;5:3;6:4;7:1;
FF 1:;2:;3:;4:PDG-REM-EEP-LMEE;5:;6:;7:;
C1 Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
   Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
   Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
   Laboratoire de Microbiologie des Environnements Extrêmes LM2E, Univ Brest, CNRS, IFREMER, IRP 1211 MicrobSea, UMR 6197, Plouzané, France
C2 RUSSIAN ACAD SCI, RUSSIA
   IFREMER, FRANCE
   CNRS, FRANCE
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 6.064
TC 5
UR https://archimer.ifremer.fr/doc/00705/81715/86315.pdf
   https://archimer.ifremer.fr/doc/00705/81715/86316.pptx
   https://archimer.ifremer.fr/doc/00705/81715/86317.xls
   https://archimer.ifremer.fr/doc/00705/81715/86318.docx
LA English
DT Article
CR BICOSE 2
BO Pourquoi pas ?
DE ;sulfate reduction;chemolithoautotroph;anaerobe;hyperthermophile;Archaea
AB Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56T (=DSM 110954T = VKM B-3474T). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56T grows lithoautotrophically with H2 as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO2/HCO3− as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO2 fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification. 
PY 2021
PD JUN
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media
VL 12
UT 000679984900001
DI 10.3389/fmicb.2021.679245
ID 81715
ER

EF