Thermococcus henrietii sp. nov., a novel extreme thermophilic and piezophilic sulfur-reducing archaeon isolated from a deep-sea hydrothermal chimney

Type Article
Date 2021
Language English
Author(s) Alain KarineORCID1, Vince Erwann2, 5, Courtine DamienORCID1, 2, 3, Maignien LoisORCID2, 5, Zeng Xiang2, 4, Shao ZongzeORCID2, 4, Jebbar MohamedORCID2, 5
Affiliation(s) 1 : Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, IUEM, Rue Dumont d’Urville, F-29280 Plouzané, France
2 : IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzané, France)-KLAMBR, Xiamen, PR China
3 : Aix Marseille Univ, CNRS, INSERM, CIML, Turing Centre for Living Systems, Marseille, France
4 : Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, PR China
5 : Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, IUEM, Rue Dumont d’Urville, F-29280 Plouzané, France
Source International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Society), 2021 , Vol. 71 , N. 7 , P. 004895 (6p.)
DOI 10.1099/ijsem.0.004895
WOS© Times Cited 1
Keyword(s) hydrothermal, thermophile, Thermococcus, piezophile
Abstract

A novel extreme thermophilic and piezophilic chemoorganoheterotrophic archaeon, strain EXT12cT, was isolated from a hydrothermal chimney sample collected at a depth of 2496 m at the East Pacific Rise 9° N. Cells were strictly anaerobic, motile cocci. The strain grew at NaCl concentrations ranging from 1 to 5 % (w/v; optimum, 2.0%), from pH 6.0 to 7.5 (optimum, pH 6.5–7.0), at temperatures between 60 and 95 °C (optimum, 80–85 °C), and at pressures from 0.1 to at least 50 MPa (optimum, 30 MPa). Strain EXT12cT grew chemoorganoheterotrophically on complex proteinaceous substrates. Its growth was highly stimulated by the presence of elemental sulphur or l-cystine, which were reduced to hydrogen sulfide. The DNA G+C content was 54.58 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated ribosomal protein sequences showed that strain EXT12cT falls into the genus Thermococcus and is most closely related to Thermococcus nautili strain 30-1T. Overall genome relatedness index analyses (average nucleotide identity scores and in silico DNA–DNA hybridizations) showed a sufficient genomic distance between the new genome and the ones of the Thermococcus type strains for the delineation of a new species. On the basis of genotypic and phenotypic data, strain EXT12cT is considered to represent a novel species, for which the name Thermococcus henrietii sp. nov. is proposed, with the type strain EXT12cT (=UBOCC M-2417T=DSM 111004T).

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Alain Karine, Vince Erwann, Courtine Damien, Maignien Lois, Zeng Xiang, Shao Zongze, Jebbar Mohamed (2021). Thermococcus henrietii sp. nov., a novel extreme thermophilic and piezophilic sulfur-reducing archaeon isolated from a deep-sea hydrothermal chimney. International Journal Of Systematic And Evolutionary Microbiology, 71(7), 004895 (6p.). Publisher's official version : https://doi.org/10.1099/ijsem.0.004895 , Open Access version : https://archimer.ifremer.fr/doc/00706/81761/