FN Archimer Export Format
PT J
TI Thermococcus henrietii sp. nov., a novel extreme thermophilic and piezophilic sulfur-reducing archaeon isolated from a deep-sea hydrothermal chimney
BT 
AF Alain, Karine
   Vince, Erwann
   Courtine, Damien
   Maignien, Lois
   Zeng, Xiang
   Shao, Zongze
   Jebbar, Mohamed
AS 1:1;2:2,5;3:1,2,3;4:2,5;5:2,4;6:2,4;7:2,5;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, IUEM, Rue Dumont d’Urville, F-29280 Plouzané, France
   IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzané, France)-KLAMBR, Xiamen, PR China
   Aix Marseille Univ, CNRS, INSERM, CIML, Turing Centre for Living Systems, Marseille, France
   Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, PR China
   Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, IUEM, Rue Dumont d’Urville, F-29280 Plouzané, France
C2 CNRS, FRANCE
   MICROBSEA, CHINA
   UNIV AIX MARSEILLE, FRANCE
   THIRD INST OCEANOG SOA, CHINA
   UBO, FRANCE
UM BEEP-LM2E
IN WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.689
TC 5
UR https://archimer.ifremer.fr/doc/00706/81761/86437.pdf
   https://archimer.ifremer.fr/doc/00706/81761/86438.pdf
LA English
DT Article
DE ;hydrothermal;thermophile;Thermococcus;piezophile
AB A novel extreme thermophilic and piezophilic chemoorganoheterotrophic archaeon, strain EXT12cT, was isolated from a hydrothermal chimney sample collected at a depth of 2496 m at the East Pacific Rise 9° N. Cells were strictly anaerobic, motile cocci. The strain grew at NaCl concentrations ranging from 1 to 5 % (w/v; optimum, 2.0%), from pH 6.0 to 7.5 (optimum, pH 6.5–7.0), at temperatures between 60 and 95 °C (optimum, 80–85 °C), and at pressures from 0.1 to at least 50 MPa (optimum, 30 MPa). Strain EXT12cT grew chemoorganoheterotrophically on complex proteinaceous substrates. Its growth was highly stimulated by the presence of elemental sulphur or l-cystine, which were reduced to hydrogen sulfide. The DNA G+C content was 54.58 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated ribosomal protein sequences showed that strain EXT12cT falls into the genus Thermococcus and is most closely related to Thermococcus nautili strain 30-1T. Overall genome relatedness index analyses (average nucleotide identity scores and in silico DNA–DNA hybridizations) showed a sufficient genomic distance between the new genome and the ones of the Thermococcus type strains for the delineation of a new species. On the basis of genotypic and phenotypic data, strain EXT12cT is considered to represent a novel species, for which the name Thermococcus henrietii sp. nov. is proposed, with the type strain EXT12cT (=UBOCC M-2417T=DSM 111004T). 
PY 2021
SO International Journal Of Systematic And Evolutionary Microbiology
SN 1466-5026
PU Microbiology Society
VL 71
IS 7
UT 000696733200023
DI 10.1099/ijsem.0.004895
ID 81761
ER

EF