FN Archimer Export Format
PT J
TI Toxicity Screening of a Gambierdiscus australes Strain from the Western Mediterranean Sea and Identification of a Novel Maitotoxin Analogue
BT 
AF Estevez, Pablo
   Castro, David
   Leão-Martins, José Manuel
   Sibat, Manoella
   Tudó, Angels
   Dickey, Robert
   Diogene, Jorge
   Hess, Philipp
   Gago-Martinez, Ana
AS 1:1;2:1;3:1;4:2;5:3;6:4;7:3;8:2;9:1;
FF 1:;2:;3:;4:PDG-ODE-DYNECO-PHYC;5:;6:;7:;8:PDG-ODE-DYNECO-PHYC;9:;
C1 Biomedical Research Centre (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain
   Laboratoire Phycotoxines, Ifremer, Rue de l’Île d’Yeu, 44311 Nantes, France
   Marine and Continental Waters Programme, Institut de Recerca i Tecnologies Agroalimentàries (IRTA), Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Spain
   Department of Marine Science, Marine Science Institute, University of Texas at Austin, Port Aransas, TX 78373, USA
C2 UNIV VIGO, SPAIN
   IFREMER, FRANCE
   IRTA, SPAIN
   UNIV TEXAS AUSTIN, USA
SI NANTES
SE PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   DOAJ
   copubli-europe
   copubli-int-hors-europe
IF 6.085
TC 16
UR https://archimer.ifremer.fr/doc/00718/83044/87991.pdf
   https://archimer.ifremer.fr/doc/00718/83044/87992.zip
LA English
DT Article
DE ;N2a;LC-MS;MS;LC-HRMS;maitotoxin;ciguatoxin;ciguatera poisoning
AB Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the identification of the compound responsible for the MTX-like toxicity in this strain. The G. australes strain was characterized not only using LC-MS instruments but also N2a-guided HPLC fractionation. Following this approach, several toxic compounds were identified in three fractions by LC-MS/MS and HRMS. A novel MTX analogue, named MTX5, was identified in the most toxic fraction, and 44-methyl gambierone and gambieric acids C and D contributed to the toxicity observed in other fractions of this strain. Thus, G. australes from the Mediterranean Sea produces MTX5 instead of MTX1 in contrast to some strains of the same species from the Pacific Ocean. No CTX precursors were detected, reinforcing the complexity of the identification of CTXs precursors in these regions 
PY 2021
PD AUG
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 19
IS 8
UT 000689378700001
DI 10.3390/md19080460
ID 83044
ER

EF