FN Archimer Export Format
PT J
TI SNP-based parentage analyses over two successive generations demonstrates the feasibility of efficient production of inbred lines in the Pacific oyster (Crassostrea gigas) by self-fertilization of simultaneous hermaphrodites despite severe inbreeding depression
BT 
AF Dégremont, Lionel
   Maurouard, Elise
   Jourdan, Antoine
   Lamy, Jean-Baptiste
   Boudry, Pierre
AS 1:1;2:1;3:1,2;4:1;5:3;
FF 1:PDG-RBE-SGMM-LGPMM;2:PDG-RBE-SGMM-LGPMM;3:;4:PDG-RBE-SGMM-LGPMM;5:PDG-RBE;
C1 Ifremer, RBE-SG2M-LGPMM, La Tremblade, France
   Sysaaf, Section Aquacole, Campus de Beaulieu, 35000 Rennes, France
   Ifremer, Département Ressources Biologiques et Environnement, Plouzané, France
C2 IFREMER, FRANCE
   SYSAAF, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   BREST
SE PDG-RBE-SGMM-LGPMM
   PDG-RBE
IN WOS Ifremer UPR
   copubli-france
IF 4.5
TC 4
UR https://archimer.ifremer.fr/doc/00722/83375/88340.pdf
LA English
DT Article
DE ;Self-fertilization;Inbreeding;Pacific oysters;Hermaphroditism;Crassostrea gigas
AB The Pacific oyster, Crassostrea gigas, is a species in which true male and true female well as hermaphrodite individuals have been reported. Among the latter, most are sequential, with one or more sex changes throughout their live, while a few (<2%) are simultaneous hermaphrodites (SH) which may self-fertilize. Sex determinism and its functional bases remain unclear is this species. In this study, one SH oyster was found among a mature broodstock and used to produce a progeny by self-fertilization. Several thousand offspring were obtained for this family (SF1) but only 73 SF1 oysters survived following an episode of mass mortality at the spat stage. Six of them were conditioned for reproduction and three were found to be SH, and one produced a limited but viable progeny by self-fertilization (SF2). Oysters were sampled and genotyped with 226 SNP markers, confirming that the SF1 and the SF2 oysters were produced by self-fertilization over two successive generations. To our knowledge, it is the second study reporting the feasibility of production on an inbred line of Pacific oysters by self-fertilization using a simultaneous hermaphrodite as primary genitor, and the first study to generate two successive generations of selfing. Observed mean inbreeding coefficient of SF2 oysters produced by selfing for two generations was 0.61, ranging from 0.46 to 0.80. This coefficient was lower than the expected theoretical value (0.8125), suggesting that the most homozygous offspring died at early stages due to inbreeding depression. Our study demonstrates that inbred lines of Pacific oysters can be produced by self-fertilization but may exhibit higher heterozygosity than theoretically expected due to better survival of the most heterozygous offspring. 
PY 2022
PD JAN
SO Aquaculture
SN 0044-8486
PU Elsevier BV
VL 547
UT 000729686800016
DI 10.1016/j.aquaculture.2021.737443
ID 83375
ER

EF