FN Archimer Export Format
PT J
TI Toward Isolation of Palytoxins: Liquid Chromatography Coupled to Low- or High-Resolution Mass Spectrometry for the Study on the Impact of Drying Techniques, Solvents and Materials
BT 
AF Mazzeo, Antonia
   Varra, Michela
   Tartaglione, Luciana
   Ciminiello, Patrizia
   ZENDONG, Zita
   HESS, Philipp
   Dell'Aversano, Carmela
AS 1:1;2:1;3:1,2;4:1;5:3;6:3;7:1,2;
FF 1:;2:;3:;4:;5:PDG-ODE-LITTORAL-PHYC;6:PDG-ODE-DYNECO-PHYC;7:;
C1 Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy
   CoNISMa—National Inter-University Consortium for Marine Sciences, Piazzale Flaminio 9, 00196 Rome, Italy
   Ifremer, DYNECO, Laboratoire Phycotoxines, Rue de l’Île d’Yeu, 44311 Nantes, France
C2 UNIV NAPLES, ITALY
   CONISMA, ITALY
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
   PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   DOAJ
   copubli-europe
IF 5.075
TC 2
UR https://archimer.ifremer.fr/doc/00722/83390/88363.pdf
   https://archimer.ifremer.fr/doc/00722/83390/88364.zip
LA English
DT Article
DE ;palytoxin;LC-HRMS;evaporation;stability;palytoxin methyl ester
AB Palytoxin (PLTX) and its congeners are emerging toxins held responsible for a number of human poisonings following the inhalation of toxic aerosols, skin contact, or the ingestion of contaminated seafood. Despite the strong structural analogies, the relative toxic potencies of PLTX congeners are quite different, making it necessary to isolate them individually in sufficient amounts for toxicological and analytical purposes. Previous studies showed poor PLTX recoveries with a dramatic decrease in PLTX yield throughout each purification step. In view of a large-scale preparative work aimed at the preparation of PLTX reference material, we have investigated evaporation as a critical—although unavoidable—step that heavily affects overall recoveries. The experiments were carried out in two laboratories using different liquid chromatography-mass spectrometry (LC-MS) instruments, with either unit or high resolution. Palytoxin behaved differently when concentrated to a minimum volume rather than when evaporated to complete dryness. The recoveries strongly depended on the solubility as well as on the material of the used container. The LC-MS analyses of PLTX dissolved in aqueous organic blends proved to give a peak intensity higher then when dissolved in pure water. After drying, the PLTX adsorption appeared stronger on glass surfaces than on plastic materials. However, both the solvents used to dilute PLTX and that used for re-dissolution had an important role. A quantitative recovery (97%) was achieved when completely drying 80% aqueous EtOH solutions of PLTX under N2-stream in Teflon. The stability of PLTX in acids was also investigated. Although PLTX was quite stable in 0.2% acetic acid solutions, upon exposure to stronger acids (pH < 2.66), degradation products were observed, among which a PLTX methyl-ester was identified 
PY 2021
PD SEP
SO Toxins
SN 2072-6651
PU MDPI
VL 13
IS 9
UT 000701088400001
DI 10.3390/toxins13090650
ID 83390
ER

EF