Amphiphilic and Perfluorinated Poly(3-Hydroxyalkanoate) Nanocapsules for 19F Magnetic Resonance Imaging

Type Article
Date 2021-09
Language English
Author(s) Le Gal Marion1, 2, Renard Estelle2, Simon-Colin Christelle1, Larrat BenoitORCID3, Langlois ValérieORCID2
Affiliation(s) 1 : Laboratoire de Microbiologie des Environnements Extrêmes, CNRS, Ifremer, University Brest, F-29280 Plouzané, France
2 : ICMPE, CNRS, University Paris Est Creteil, F-94010 Creteil, France
3 : Université Paris-Saclay, CEA, CNRS, NeuroSpin, 91191 Gif-sur-Yvette, France
Source Bioengineering-basel (2306-5354) (MDPI AG), 2021-09 , Vol. 8 , N. 9 , P. 121 (15p.)
DOI 10.3390/bioengineering8090121
WOS© Times Cited 2
Note This article belongs to the Special Issue Magnetic Micronanotechnologies for Bioengineering Applications
Keyword(s) polyhydroxyalkanoates, mcl-PHA, nanocapsules, fluorinated polymer, amphiphilic copolymer
Abstract

Nanoparticles have recently emerged as valuable tools in biomedical imaging techniques. Here PEGylated and fluorinated nanocapsules based on poly(3-hydroxyalkanoate) containing a liquid core of perfluorooctyl bromide PFOB were formulated by an emulsion-evaporation process as potential 19F MRI imaging agents. Unsaturated poly(hydroxyalkanoate), PHAU, was produced by marine bacteria using coprah oil and undecenoic acid as substrates. PHA-g-(F; PEG) was prepared by two successive controlled thiol-ene reactions from PHAU with firstly three fluorinated thiols having from 3 up to 17 fluorine atoms and secondly with PEG-SH. The resulting PHA-g-(F; PEG)-based PFOB nanocapsules, with a diameter close to 250–300 nm, are shown to be visible in 19F MRI with an acquisition time of 15 min. The results showed that PFOB-nanocapsules based on PHA-g-(F; PEG) have the potential to be used as novel contrast agents for 19F MRI.

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