FN Archimer Export Format
PT J
TI Quorum sensing disruption regulates hydrolytic enzyme and biofilm production in estuarine bacteria
BT 
AF URVOY, Marion
   LAMI, Raphael
   DREANNO, Catherine
   Daudé, David
   Rodrigues, Alice M S
   GOURMELON, Michele
   L'HELGUEN, Stephane
   LABRY, Claire
AS 1:1,2;2:3;3:5;4:4;5:3;6:1;7:2;8:1;
FF 1:;2:;3:PDG-REM-RDT-LDCM;4:;5:;6:PDG-ODE-DYNECO-PELAGOS;7:;8:PDG-ODE-DYNECO-PELAGOS;
C1 Ifremer, DYNECO, F-29280 Plouzané, France
   Université de Bretagne Occidentale, CNRS, IRD, Ifremer, UMR 6539, Laboratoire des Sciences de l’Environnement Marin (LEMAR), F-29280 Plouzané, France
   Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), 66650 Banyuls-sur-Mer, France
   Gene&GreenTK, 19-21 Boulevard Jean Moulin, 13005 Marseille, France
   Ifremer, RDT, F-29280 Plouzané, France
C2 IFREMER, FRANCE
   UBO, FRANCE
   UNIV SORBONNE, FRANCE
   GENE&GREENTK, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
   PDG-REM-RDT-LDCM
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 5.476
TC 6
UR https://archimer.ifremer.fr/doc/00723/83499/88506.pdf
LA English
DT Article
AB Biofilms of heterotrophic bacteria cover organic matter aggregates and constitute hotspots of mineralization, primarily acting through extracellular hydrolytic enzyme production. Nevertheless, regulation of both biofilm and hydrolytic enzyme synthesis remains poorly investigated, especially in estuarine ecosystems. In this study, various bioassays, mass spectrometry and genomics approaches were combined to test the possible involvement of quorum sensing (QS) in these mechanisms. QS is a bacterial cell-cell communication system that relies notably on the emission of N-acylhomoserine lactones (AHLs). In our estuarine bacterial collection, we found that 28 strains (9%), mainly Vibrio, Pseudomonas and Acinetobacter isolates, produced at least 14 different types of AHLs encoded by various luxI genes. We then inhibited the AHL QS circuits of those 28 strains using a broad-spectrum lactonase preparation and tested whether biofilm production as well as β-glucosidase and leucineaminopeptidase activities were impacted. Interestingly, we recorded contrasted responses, as biofilm production, dissolved and cell-bound β-glucosidase and leucine-aminopeptidase activities significantly increased in 4%-68% of strains but decreased in 0%-21% of strains. These findings highlight the key role of AHL-based QS in estuarine bacterial physiology and ultimately on biogeochemical cycles. They also point out the complexity of QS regulations within natural microbial assemblages. 
PY 2021
PD NOV
SO Environmental Microbiology
SN 1462-2912
PU Wiley / Blackwell
VL 23
IS 11
UT 000707649700001
BP 7183
EP 7200
DI 10.1111/1462-2920.15775
ID 83499
ER

EF