FN Archimer Export Format PT J TI Development of an exposure protocol for toxicity test (FEET) for a marine species: the European sea bass (Dicentrarchus labrax) BT AF Soloperto, Sofia Aroua, Salima Jozet-Alves, Christelle Minier, Christophe Halm-Lemeille, Marie-Pierre AS 1:1;2:1;3:2,3;4:1;5:4; FF 1:;2:;3:;4:;5:PDG-ODE-LITTORAL; C1 UMR-I 02 SEBIO - Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université du Havre, 25, Rue Philippe Lebon, 76600, Le Havre, France Unicaen, CNRS, Normandie Univ, 14000, Caen, France EthoS (Éthologie animale et humaine) - UMR 6552, Univ Rennes, CNRS, F-35000, Rennes, France Unité Littoral Ifremer, LITTORAL, F-14520, Port-en-Bessin, France C2 UNIV LE HAVRE, FRANCE UNIV CAEN NORMANDIE, FRANCE UNIV RENNES, FRANCE IFREMER, FRANCE SI PORT-EN-BESSIN SE PDG-ODE-LITTORAL IN WOS Ifremer UPR copubli-france copubli-univ-france IF 5.8 TC 2 UR https://archimer.ifremer.fr/doc/00729/84055/89054.pdf LA English DT Article DE ;Early-life stage toxicity test;Temperate conditions;Sea bass larvae;3;4-Dichloroaniline;Behavioral test;96 h LC50 AB Regulatory assessment of the effects of chemicals requires the availability of validated tests representing different environments and organisms. In this context, developing new tests is particularly needed for marine species from temperate environments. It is also important to evaluate effects that are generally poorly characterized and seldom included in regulatory tests. In this study, we designed an exposure protocol using European sea bass (Dicentrarchus labrax) larvae. We examined classical toxicological values (LCx) as well as behavioral responses. By comparing different hatching and breeding strategies, we defined the optimal conditions of exposure as non-agitated conditions in 24- or 48-well microplates. Our exposure protocol was then tested with 3,4-dichloroaniline (3,4-DCA), a recommended reference molecule. Based on our results, the 96 h LC50 for 3,4-DCA corresponded to 2.04 mg/L while the 168 h LC50 to 0.79 mg/L. Behavioral analyses showed no effect of 3,4-DCA at low concentration (0.25 mg/L). In conclusion, the present work established the basis for a new test which includes behavioral analysis and shows that the use of sea bass is suitable to early-life stage toxicity tests. PY 2022 PD MAR SO Environmental Science And Pollution Research SN 0944-1344 PU Springer Science and Business Media LLC VL 29 IS 11 UT 000706053800017 BP 15777 EP 15790 DI 10.1007/s11356-021-16785-z ID 84055 ER EF