FN Archimer Export Format
PT J
TI The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor
BT 
AF Luviano, Nelia
   Lopez, Marie
   Gawehns, Fleur
   Chaparro, Cristian
   Arimondo, Paola B.
   Ivanovic, Slavica
   David, Patrice
   Verhoeven, Koen
   Cosseau, Céline
   Grunau, Christoph
AS 1:1;2:2,3;3:4;4:1;5:3,5;6:6;7:7;8:6;9:1;10:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 IHPE, Univ Perpignan Via Domitia, CNRC, Ifremer, Univ Montpellier, Perpignan, France
   Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, Univ Montpellier, ENSCM, Montpellier, France
   Epigenetic Targeting of Cancer (ETaC), CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France
   Bioinformatics Unit, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands
   Epigenetic Chemical Biology (EpiChBio), Department Structural Biology and Chemistry, UMR 3523, CNRS, Institute Pasteur, 75015, Paris, France
   Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands
   Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Univ. Montpellier, CNRS - Université Paul Valéry Montpellier - EPHE, 1919 Route de Mende, 34293, Montpellier Cedex 5, France
C2 UNIV PERPIGNAN, FRANCE
   UNIV MONTPELLIER, FRANCE
   CNRS, FRANCE
   NIOO-KNAW, NETHERLANDS
   CNRS, FRANCE
   NIOO-KNAW, NETHERLANDS
   UNIV MONTPELLIER, FRANCE
UM IHPE
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 5.488
TC 7
UR https://archimer.ifremer.fr/doc/00732/84361/89368.pdf
   https://archimer.ifremer.fr/doc/00732/84361/89369.xlsx
LA English
DT Article
DE ;DNMT inhibitors;5-Methylcytosine;Mollusks;Invertebrates;Epigenetic inheritance;Epimutation
AB Background 5-Methylcytosine (5mC) is an important epigenetic mark in eukaryotes. Little information about its role exists for invertebrates. To investigate the contribution of 5mC to phenotypic variation in invertebrates, alteration of methylation patterns needs to be produced. Here, we apply new non-nucleoside DNA methyltransferase inhibitors (DNMTi) to introduce aleatory changes into the methylome of mollusk species. Results Flavanone inhibitor Flv1 was efficient in reducing 5mC in the freshwater snails Biomphalaria glabrata and Physa acuta, and to a lesser degree, probably due to lower stability in sea water, in the oyster Crassostrea gigas. Flv1 has no toxic effects and significantly decreased the 5mC level in the treated B. glabrata and in its offspring. Drug treatment triggers significant variation in the shell height in both generations. A reduced representation bisulfite-sequencing method called epiGBS corroborates hypomethylation effect of Flv1 in both B. glabrata generations and identifies seven Differential Methylated Regions (DMR) out of 32 found both in Flv1-exposed snails and its progeny, from which 5 were hypomethylated, demonstrating a multigenerational effect. By targeted bisulfite sequencing, we confirmed hypomethylation in a locus and show that it is associated with reduced gene expression. Conclusions Flv1 is a new and efficient DNMTi that can be used to induce transient and heritable modifications of the epigenetic landscape and phenotypic traits in mollusks, a phylum of the invertebrates in which epigenetics is understudied. 
PY 2021
PD OCT
SO Epigenetics & Chromatin
SN 1756-8935
PU Springer Science and Business Media LLC
VL 14
IS 1
UT 000711429200001
DI 10.1186/s13072-021-00422-7
ID 84361
ER

EF