TY - JOUR T1 - A High Rate Algal Pond Hosting a Dynamic Community of RNA Viruses A1 - Chase,Emily E. A1 - Monteil-Bouchard,Sonia A1 - Gobet,Angelique A1 - Andrianjakarivony,Felana H. A1 - Desnues,Christelle A1 - Blanc,Guillaume AD - Microbiologie Environnementale Biotechnologie, Institut Méditerranéen d’Océanologie, 163 Avenue de Luminy, 13009 Marseille, France AD - Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France AD - MARBEC University Montpellier, CNRS, Ifremer, IRD, 34203 Sète, France UR - https://archimer.ifremer.fr/doc/00732/84416/ DO - 10.3390/v13112163 KW - microalgae KW - Marnaviridae KW - community diversity KW - community dynamics N2 - Despite a surge of RNA virome sequencing in recent years, there are still many RNA viruses to uncover—as indicated by the relevance of viral dark matter to RNA virome studies (i.e., putative viruses that do not match to taxonomically identified viruses). This study explores a unique site, a high-rate algal pond (HRAP), for culturing industrially microalgae, to elucidate new RNA viruses. The importance of viral-host interactions in aquatic systems are well documented, and the ever-expanding microalgae industry is no exception. As the industry becomes a more important source of sustainable plastic manufacturing, a producer of cosmetic pigments and alternative protein sources, and a means of CO2 remediation in the face of climate change, studying microalgal viruses becomes a vital practice for proactive management of microalgae cultures at the industrial level. This study provides evidence of RNA microalgal viruses persisting in a CO2 remediation pilot project HRAP and uncovers the diversity of the RNA virosphere contained within it. Evidence shows that family Marnaviridae is cultured in the basin, alongside other potential microalgal infecting viruses (e.g., family Narnaviridae, family Totitiviridae, and family Yueviridae). Finally, we demonstrate that the RNA viral diversity of the HRAP is temporally dynamic across two successive culturing seasons. Y1 - 2021/11 PB - MDPI AG JF - Viruses-basel SN - 1999-4915 VL - 13 IS - 11 ID - 84416 ER -