FN Archimer Export Format
PT J
TI Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
BT 
AF Boyd, Bret M.
   Chevignon, Germain
   Patel, Vilas
   Oliver, Kerry M.
   Strand, Michael R.
AS 1:1;2:2;3:1;4:1;5:1;
FF 1:;2:;3:;4:;5:;
C1 Department of Entomology, University of Georgia Athens, Athens, GA, USA
   Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, La Tremblade, France
C2 UNIV ATHENS, USA
   Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, La Tremblade, France
IN DOAJ
IF 5.916
TC 10
UR https://archimer.ifremer.fr/doc/00733/84530/89624.pdf
   https://archimer.ifremer.fr/doc/00733/84530/89625.pdf
LA English
DT Article
DE ;Virus;Bacteria;Mutualism;Aphid;Parasitoid
AB Background Most phages infect free-living bacteria but a few have been identified that infect heritable symbionts of insects or other eukaryotes. Heritable symbionts are usually specialized and isolated from other bacteria with little known about the origins of associated phages. Hamiltonella defensa is a heritable bacterial symbiont of aphids that is usually infected by a tailed, double-stranded DNA phage named APSE. Methods We conducted comparative genomic and phylogenetic studies to determine how APSE is related to other phages and prophages. Results Each APSE genome was organized into four modules and two predicted functional units. Gene content and order were near-fully conserved in modules 1 and 2, which encode predicted DNA metabolism genes, and module 4, which encodes predicted virion assembly genes. Gene content of module 3, which contains predicted toxin, holin and lysozyme genes differed among haplotypes. Comparisons to other sequenced phages suggested APSE genomes are mosaics with modules 1 and 2 sharing similarities with Bordetella-Bcep-Xylostella fastidiosa-like podoviruses, module 4 sharing similarities with P22-like podoviruses, and module 3 sharing no similarities with known phages. Comparisons to other sequenced bacterial genomes identified APSE-like elements in other heritable insect symbionts (Arsenophonus spp.) and enteric bacteria in the family Morganellaceae. Conclusions APSEs are most closely related to phage elements in the genus Arsenophonus and other bacteria in the Morganellaceae. 
PY 2021
PD NOV
SO Virology Journal
SN 1743-422X
PU Springer Science and Business Media LLC
VL 18
IS 1
UT 000716931600001
DI 10.1186/s12985-021-01685-y
ID 84530
ER

EF