FN Archimer Export Format
PT J
TI Metal stresses modify soluble proteomes and toxin profiles in two Mediterranean strains of the distributed dinoflagellate Alexandrium pacificum
BT 
AF Jean, Natacha
   Perié, Luce
   Dumont, Estelle
   Bertheau, Lucie
   Balliau, Thierry
   Caruana, Amandine
   Amzil, Zouher
   Laabir, Mohamed
   Masseret, Estelle
AS 1:1;2:2;3:3;4:5;5:4;6:6;7:6;8:7;9:7;
FF 1:;2:;3:;4:;5:;6:PDG-ODE-PHYTOX-PHYSALG;7:PDG-ODE-PHYTOX-METALG;8:;9:;
C1 Université de Toulon, Aix Marseille Univ, CNRS, IRD, MIO, Toulon, France
   Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, New York University, 30th St., New York, NY 10016, USA
   UMR_MD1, Aix-Marseille Univ, U-1261-INSERM, SSA, IRBA, MCT, Marseille, France
   PAPPSO-GQE-Le Moulon, INRA, Université Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91 190 Gif-sur-Yvette, France
   UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, Université de Bourgogne Franche-Comté, AgroSup Dijon, esplanade Erasme, 21 000 Dijon, France
   IFREMER, Phycotoxin Laboratory, rue de l'île d'Yeu, BP 21105, 44 311 Nantes, France
   Marbec, Univ Montpellier, IRD, Ifremer, CNRS, Montpellier, France
C2 UNIV TOULON, FRANCE
   UNIV NEW YORK, USA
   UNIV AIX MARSEILLE, FRANCE
   UNIV PARIS SUD, FRANCE
   UNIV BOURGOGNE FRANCHE COMTE, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
SI NANTES
SE PDG-ODE-PHYTOX-PHYSALG
   PDG-ODE-PHYTOX-METALG
UM MARBEC
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 9.8
TC 7
UR https://archimer.ifremer.fr/doc/00735/84659/89752.pdf
LA English
DT Article
DE ;Harmful algal bloom;Paralytic shellfish toxins;Proteomics;Metals
AB HABs involving Alexandrium pacificum have been reported in metal-contaminated ecosystems, suggesting that this distributed species adapts to and/or can tolerate the effects of metals. Modifications in soluble proteomes and PST contents were characterized in two Mediterranean A. pacificum strains exposed to mono- or polymetallic stresses (zinc, lead, copper, cadmium). These strains were isolated from two anthropized locations: Santa Giusta Lagoon (Italy, SG C10–3) and the Tarragona seaport (Spain, TAR C54F). In both strains, metals primarily downregulated key photosynthesis proteins. Metals also upregulated other proteins involved in photosynthesis (PCP in both strains), the oxidative stress response (HSP 60, proteasome and SOD in SG C10–3; HSP 70 in TAR C54F), energy metabolism (AdK in TAR C54F), neoglucogenesis/glycolysis (GAPDH and PEP synthase in SG C10–3) and protein modification (PP in TAR C54F). These proteins, possibly involved in adaptive proteomic responses, may explain the development of these A. pacificum strains in metal-contaminated ecosystems. The two strains showed different proteomic responses to metals, with SG C10–3 upregulating more proteins, particularly PCP. Among the PSTs, regardless of the metal and the strain studied, C2 and GTX4 predominated, followed by GTX5. Under the polymetallic cocktail, (i) total PSTs, C2 and GTX4 reached the highest levels in SG C10–3 only, and (ii) total PSTs, C2, GTX5 and neoSTX were higher in SG C10–3 than in TAR C54F, whereas in SG C10–3 under copper stress, total PSTs, GTX5, GTX1 and C1 were higher than in the controls, revealing variability in PST biosynthesis between the two strains. Total PSTs, C2, GTX4 and GTX1 showed significant positive correlations with PCP, indicating that PST production may be positively related to photosynthesis. Our results showed that the A. pacificum strains adapt their proteomic and physiological responses to metals, which may contribute to their ecological success in highly anthropized areas. 
PY 2022
PD APR
SO Science Of The Total Environment
SN 0048-9697
PU Elsevier BV
VL 818
UT 000789651000009
DI 10.1016/j.scitotenv.2021.151680
ID 84659
ER

EF