FN Archimer Export Format
PT J
TI SARS-CoV-2 genome quantification in wastewaters at regional and city scale allows precise monitoring of the whole outbreaks dynamics and variants spreading in the population
BT 
AF Wurtzer, S.
   Waldman, P.
   Levert, M.
   Cluzel, N.
   Almayrac, J.L.
   Charpentier, C.
   Masnada, F.
   Gillon-Ritz, M.
   Mouchel, J.M.
   Maday, Y.
   Boni, M.
   Marechal, V
   Moulin, L
AS 1:1;2:2;3:3;4:3;5:4;6:5;7:6;8:7;9:3;10:3;11:8;12:;13:;14:9;15:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;
C1 Eau de Paris, R&D Laboratory, DRDQE Ivry/Seine, France
   Sorbonne Université, CNRS, EPHE, UMR 7619 Metis, e-LTER Zone Atelier Seine, Paris, France
   Sorbonne Université, CNRS, Université de Paris, Laboratoire Jacques-Louis Lions (LJLL), et Institut Universitaire de France, Paris, France
   SIAAP, Service process-laboratoire SIAAP site Seine Amont, Valenton, France
   Service de Virologie, Université de Paris, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France
   SIAM – STV, Avenue de la courtiere, 77400 Saint Thibault des vignes, France
   Direction de la Propreté et de l'Eau - Service Technique de l'Eau et de l'Assainissement, rue du Commandeur, 75014 Paris, France
   Institut de Recherche Biomédicale des Armées, 1 place Valérie André, F-91220 Brétigny sur Orge, France
   Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine2, Paris, France
C2 EAU DE PARIS, FRANCE
   UNIV SORBONNE, FRANCE
   UNIV SORBONNE, FRANCE
   SIAAP, FRANCE
   INSERM, FRANCE
   SIAM – STV, FRANCE
   DIRECT PROPRETE EAU, FRANCE
   INST RECH BIOMED ARMEES IRBA, FRANCE
   UNIV SORBONNE, FRANCE
SI NANTES
SE PDG-RBE-MASAE-LSEM
IF 9.8
TC 37
UR https://archimer.ifremer.fr/doc/00740/85166/90155.pdf
LA English
DT Article
DE ;SARS-CoV-2;COVID-19;Quantification;Wastewater;Variant monitoring;RT-qPCR;Epidemiology
AB SARS-CoV-2 is a coronavirus causing a globalized outbreak called COVID-19. SARS-CoV-2 transmission is associated with inhalation of contaminated respiratory droplets and could causes severe complications. Until today several “waves” of infections have been observed despite implementation of strict health policies. Decisions for such sanitary measures are based on population health monitoring. Unfortunately, for COVID-19, a significant proportion of individuals are asymptomatic but play a role in the virus transmission. To overcome these limitations, several strategies were developed including genome quantification in wastewater that could allow monitoring of the health status of population, since shedding of SARS-CoV-2 in patient stool is frequent. Wastewater-based epidemiology (WBE) was established and several countries implemented this approach to allow COVID-19 outbreak monitoring. In France, the OBEPINE project performed a quantitative analysis of SARS-CoV-2 in raw wastewater samples collected from major wastewater treatment plants (WWTP) since March 2020. In the greater Paris area 1101 samples (507 for five WWTP and 594 for sewer) were collected. This 16 months monitoring allows us to observe the outbreak dynamics. Comparison of WBE indicators with health data lead to several important observation; the good level of correlation with incidence rates, the average 3 days lead time, and the sensitivity (WBE change when incidence is > to 7/100000 inhabitants). We also compared the local monitoring (city level) with the regional monitoring, to help cluster identification. Moreover, variants of concern (VOC) emerged due to the selection pressure. We developed a specific RT-qPCR method targeting the deletion H69-V70 in the spike protein, using this deletion as a proxy of the B.1.1.7 presence in the wastewater. With this data we demonstrate the predominant role played by this strain in the third wave. All these results allow a better description and understanding of the pandemic and highlight the role of such WBE indicators. 
PY 2022
PD MAR
SO Science Of The Total Environment
SN 0048-9697
PU Elsevier BV
VL 810
UT 000744495400014
DI 10.1016/j.scitotenv.2021.152213
ID 85166
ER

EF