FN Archimer Export Format PT J TI Unraveling the genotype by environment interaction in a thermosensitive fish with a polygenic sex determination system BT AF Geffroy, Benjamin Besson, Mathieu Sánchez-Baizán, Núria Clota, Frederic Goikoetxea, Alexander Sadoul, Bastien Ruelle, Francois BLANC, Marie-Odile Parrinello, Hugues Hermet, Sophie Blondeau-Bidet, Eva Pratlong, Marine Piferrer, Francesc Vandeputte, Marc Allal, Francois AS 1:1;2:2,3;3:4;4:1,3;5:1;6:1,5;7:6;8:6;9:7;10:8;11:8;12:7;13:4;14:1,3;15:1; FF 1:PDG-RBE-MARBEC-LAAAS;2:;3:;4:;5:PDG-RBE-MARBEC-LAAAS;6:PDG-RBE-MARBEC-LAAAS;7:PDG-RBE-MARBEC-LSEA;8:PDG-RBE-MARBEC-LSEA;9:;10:;11:;12:;13:;14:;15:PDG-RBE-MARBEC-LAAAS; C1 MARBEC Université de Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France SYSAAF, Station LPGP/INRAE, 35042 Rennes, France Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France Institut de Ciències del Mar, Spanish National Research Council, Barcelona, Spain ESE, Ecology and Ecosystem Health, Institut Agro, INRAE, Rennes, France Laboratoire Service d'Expérimentations Aquacoles, Ifremer, Palavas-les-Flots, France MGX, BCM, Université de Montpellier, CNRS, INSERM, Montpellier, France MARBEC Université de Montpellier, CNRS, Ifremer, IRD, Montpellier, France C2 IFREMER, FRANCE SYSAAF, FRANCE INRAE, FRANCE ICM CSIC, SPAIN INRAE, FRANCE IFREMER, FRANCE UNIV MONTPELLIER, FRANCE UNIV MONTPELLIER, FRANCE SI PALAVAS SE PDG-RBE-MARBEC-LAAAS PDG-RBE-MARBEC-LSEA UM MARBEC IN WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-p187 copubli-europe copubli-univ-france IF 12.779 TC 15 UR https://archimer.ifremer.fr/doc/00740/85205/90215.pdf https://archimer.ifremer.fr/doc/00740/85205/90217.pdf https://archimer.ifremer.fr/doc/00740/85205/90218.txt https://archimer.ifremer.fr/doc/00740/85205/90219.csv https://archimer.ifremer.fr/doc/00740/85205/90220.txt https://archimer.ifremer.fr/doc/00740/85205/90221.txt LA English DT Article DE ;sex determination;genomics;temperature;fish;epigenetic AB In most animals, sex determination occurs at conception, when sex chromosomes are segregated following Mendelian laws. However, in multiple reptiles and fishes, this genetic sex can be overridden by external factors after fertilization or birth. In some species, the genetic sex may also be governed by multiple genes, further limiting our understanding of sex determination in such species. We used the European sea bass (Dicentrarchus labrax) as a model and combined genomic (using a single nucleotide polymorphism chip) and transcriptomic (RNA-Sequencing) approaches to thoroughly depict this polygenic sex determination system and its interaction with temperature. We estimated genetic sex tendency (eGST), defined as the estimated genetic liability to become a given sex under a liability threshold model for sex determination, which accurately predicts the future phenotypic sex. We found evidence that energetic pathways, concerning the regulation of lipids and glucose, are involved in sex determination and could explain why females tend to exhibit higher energy levels and improved growth compared to males. Besides, early exposure to high-temperature up-regulated sox3, followed by sox9a in individuals with intermediate eGST, but not in individuals showing highly female-biased eGST, providing the most parsimonious explanation for temperature-induced masculinization. This gonadal state was maintained likely by DNA methylation and the up-regulation of several genes involved in histone modifications, including jmjd1c. Overall, we describe a sex determination system resulting from continuous genetic and environmental influences in an animal. Our results provide significant progress in our understanding of the mechanisms underlying temperature-induced masculinization in fish. PY 2021 PD DEC SO Proceedings Of The National Academy Of Sciences Of The United States Of America SN 0027-8424 PU Proceedings of the National Academy of Sciences VL 118 IS 50 UT 000732715700037 DI 10.1073/pnas.2112660118 ID 85205 ER EF