Pituitary Hormones mRNA Abundance in the Mediterranean Sea Bass Dicentrarchus labrax: Seasonal Rhythms, Effects of Melatonin and Water Salinity

Type Article
Date 2021-12
Language English
Author(s) Falcón Jack1, 2, Herrero Maria Jesus2, Nisembaum Laura Gabriela2, 3, Isorna Esther3, Peyric Elodie2, Beauchaud Marilyn4, Attia Joël4, Covès Denis5, Fuentès Michael2, Delgado Maria Jesus3, Besseau Laurence2
Affiliation(s) 1 : Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), MNHN, CNRS UMR 8067, SU, IRD 207, UCN, UA, Paris, France
2 : Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Banyuls-sur-Mer, France
3 : Department of Genetics, Physiology and Microbiology, Complutense University of Madrid (UCM), Madrid, Spain
4 : Equipe de Neuro-Ethologie Sensorielle, ENES/CRNL, CNRS UMR 5292, UMR-S 1028, Faculté des Sciences et Techniques, Université Jean-Monnet (UJM), Saint-Étienne, France
5 : Station Ifremer de Palavas, Palavas-les-Flots, Nantes, France
Source Frontiers In Physiology (1664-042X) (Frontiers Media SA), 2021-12 , Vol. 12 , P. 774975 (13p.)
DOI 10.3389/fphys.2021.774975
Keyword(s) sea bass, pituitary, hormones, annual variations, melatonin, photoperiod, salinity
Abstract

In fish, most hormonal productions of the pituitary gland display daily and/or seasonal rhythmic patterns under control by upstream regulators, including internal biological clocks. The pineal hormone melatonin, one main output of the clocks, acts at different levels of the neuroendocrine axis. Melatonin rhythmic production is synchronized mainly by photoperiod and temperature. Here we aimed at better understanding the role melatonin plays in regulating the pituitary hormonal productions in a species of scientific and economical interest, the euryhaline European sea bass Dicentrarchus labrax. We investigated the seasonal variations in mRNA abundance of pituitary hormones in two groups of fish raised one in sea water (SW fish), and one in brackish water (BW fish). The mRNA abundance of three melatonin receptors was also studied in the SW fish. Finally, we investigated the in vitro effects of melatonin or analogs on the mRNA abundance of pituitary hormones at two times of the year and after adaptation to different salinities. We found that (1) the reproductive hormones displayed similar mRNA seasonal profiles regardless of the fish origin, while (2) the other hormones exhibited different patterns in the SW vs. the BW fish. (3) The melatonin receptors mRNA abundance displayed seasonal variations in the SW fish. (4) Melatonin affected mRNA abundance of most of the pituitary hormones in vitro; (5) the responses to melatonin depended on its concentration, the month investigated and the salinity at which the fish were previously adapted. Our results suggest that the productions of the pituitary are a response to multiple factors from internal and external origin including melatonin. The variety of the responses described might reflect a high plasticity of the pituitary in a fish that faces multiple external conditions along its life characterized by marked daily and seasonal changes in photoperiod, temperature and salinity.

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Falcón Jack, Herrero Maria Jesus, Nisembaum Laura Gabriela, Isorna Esther, Peyric Elodie, Beauchaud Marilyn, Attia Joël, Covès Denis, Fuentès Michael, Delgado Maria Jesus, Besseau Laurence (2021). Pituitary Hormones mRNA Abundance in the Mediterranean Sea Bass Dicentrarchus labrax: Seasonal Rhythms, Effects of Melatonin and Water Salinity. Frontiers In Physiology, 12, 774975 (13p.). Publisher's official version : https://doi.org/10.3389/fphys.2021.774975 , Open Access version : https://archimer.ifremer.fr/doc/00740/85247/