The mosquito microbiome includes habitat-specific but rare symbionts

Type Article
Date 2022
Language English
Author(s) Schrieke Hans1, Maignien Lois2, Constancias Florentin3, Trigodet Florian4, Chakloute Sarah5, Rakotoarivony Ignace5, Marie Albane6, L'Ambert Gregory6, Makoundou Patrick7, Pages Nonito5, 8, Murat Eren A.4, 9, Weill Mylène8, Sicard Mathieu8, Reveillaud Julie1
Affiliation(s) 1 : MIVEGEC, University of Montpellier, INRAE, CNRS, IRD, Montpellier, France
2 : Univ Brest, CNRS, IFREMER, Microbiology of Extreme Environments Laboratory, Plouzané, France
3 : CIRAD, UMR Qualisud, Montpellier, France
4 : Department of Medicine, University of Chicago, Chicago, IL, USA
5 : ASTRE, University of Montpellier, CIRAD, INRAE, Montpellier, France
6 : EID Méditerranée, 165 avenue Paul Rimbaud, 34184 Montpellier, France
7 : ISEM, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France
8 : CIRAD, UMR ASTRE, Guadeloupe, France
9 : Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, USA
Source Computational And Structural Biotechnology Journal (2001-0370) (Elsevier BV), 2022 , Vol. 20 , P. 410-420
DOI 10.1016/j.csbj.2021.12.019
WOS© Times Cited 3
Keyword(s) Microbiome, Mosquitoes, Symbionts, Microdiversity, Oligotyping

Microbial communities are known to influence mosquito lifestyles by modifying essential metabolic and behavioral processes that affect reproduction, development, immunity, digestion, egg survival, and the ability to transmit pathogens. Many studies have used 16S rRNA gene amplicons to characterize mosquito microbiota and investigate factors that influence host-microbiota dynamics. However, a relatively low taxonomic resolution due to clustering methods based on arbitrary threshold and the overall dominance of Wolbachia or Asaia symbionts obscured the investigation of rare members of mosquito microbiota in previous studies. Here, we used high resolution Shannon entropy-based oligotyping approach to analyze the microbiota of Culex pipiens, Culex quinquefasciatus and Aedes individuals from continental Southern France and overseas Guadeloupe as well as from laboratories with or without antibiotics treatment. Our experimental design that resulted in a series of mosquito samples with a gradient of Wolbachia density and relative abundance along with high-resolution analyses of amplicon sequences enabled the recovery of a robust signal from typically less accessible bacterial taxa. Our data confirm species-specific mosquito-bacteria associations with geography as a primary factor that influences bacterial community structure. But interestingly, they also reveal co-occurring symbiotic bacterial variants within single individuals for both Elizabethkingia and Erwinia genera, distinct and specific Asaia and Chryseobacterium in continental and overseas territories, and a putative rare Wolbachia variant. Overall, our study reveals the presence of previously overlooked microdiversity and multiple closely related symbiotic strains within mosquito individuals with a remarkable habitat-specificity.

Full Text
File Pages Size Access
Publisher's official version 25 1 MB Open access
Supplementary data 1. 24 41 MB Open access
Top of the page

How to cite 

Schrieke Hans, Maignien Lois, Constancias Florentin, Trigodet Florian, Chakloute Sarah, Rakotoarivony Ignace, Marie Albane, L'Ambert Gregory, Makoundou Patrick, Pages Nonito, Murat Eren A., Weill Mylène, Sicard Mathieu, Reveillaud Julie (2022). The mosquito microbiome includes habitat-specific but rare symbionts. Computational And Structural Biotechnology Journal, 20, 410-420. Publisher's official version : , Open Access version :