FN Archimer Export Format
PT J
TI The mosquito microbiome includes habitat-specific but rare symbionts
BT 
AF Schrieke, Hans
   Maignien, Lois
   Constancias, Florentin
   Trigodet, Florian
   Chakloute, Sarah
   Rakotoarivony, Ignace
   Marie, Albane
   L'Ambert, Gregory
   Makoundou, Patrick
   Pages, Nonito
   Murat Eren, A.
   Weill, Mylène
   Sicard, Mathieu
   Reveillaud, Julie
AS 1:1;2:2;3:3;4:4;5:5;6:5;7:6;8:6;9:7;10:5,8;11:4,9;12:8;13:8;14:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;
C1 MIVEGEC, University of Montpellier, INRAE, CNRS, IRD, Montpellier, France
   Univ Brest, CNRS, IFREMER, Microbiology of Extreme Environments Laboratory, Plouzané, France
   CIRAD, UMR Qualisud, Montpellier, France
   Department of Medicine, University of Chicago, Chicago, IL, USA
   ASTRE, University of Montpellier, CIRAD, INRAE, Montpellier, France
   EID Méditerranée, 165 avenue Paul Rimbaud, 34184 Montpellier, France
   ISEM, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France
   CIRAD, UMR ASTRE, Guadeloupe, France
   Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, USA
C2 UNIV MONTPELLIER, FRANCE
   UBO, FRANCE
   CIRAD, FRANCE
   UNIV CHICAGO, USA
   UNIV MONTPELLIER, FRANCE
   EID MEDITERRANEE, FRANCE
   UNIV MONTPELLIER, FRANCE
   CIRAD, FRANCE
   MARINE BIOLOGICAL LAB, USA
UM BEEP-LM2E
IN WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 6
TC 9
UR https://archimer.ifremer.fr/doc/00741/85332/90359.pdf
   https://archimer.ifremer.fr/doc/00741/85332/90637.pdf
LA English
DT Article
DE ;Microbiome;Mosquitoes;Symbionts;Microdiversity;Oligotyping
AB Microbial communities are known to influence mosquito lifestyles by modifying essential metabolic and behavioral processes that affect reproduction, development, immunity, digestion, egg survival, and the ability to transmit pathogens. Many studies have used 16S rRNA gene amplicons to characterize mosquito microbiota and investigate factors that influence host-microbiota dynamics. However, a relatively low taxonomic resolution due to clustering methods based on arbitrary threshold and the overall dominance of Wolbachia or Asaia symbionts obscured the investigation of rare members of mosquito microbiota in previous studies. Here, we used high resolution Shannon entropy-based oligotyping approach to analyze the microbiota of Culex pipiens, Culex quinquefasciatus and Aedes individuals from continental Southern France and overseas Guadeloupe as well as from laboratories with or without antibiotics treatment. Our experimental design that resulted in a series of mosquito samples with a gradient of Wolbachia density and relative abundance along with high-resolution analyses of amplicon sequences enabled the recovery of a robust signal from typically less accessible bacterial taxa. Our data confirm species-specific mosquito-bacteria associations with geography as a primary factor that influences bacterial community structure. But interestingly, they also reveal co-occurring symbiotic bacterial variants within single individuals for both Elizabethkingia and Erwinia genera, distinct and specific Asaia and Chryseobacterium in continental and overseas territories, and a putative rare Wolbachia variant. Overall, our study reveals the presence of previously overlooked microdiversity and multiple closely related symbiotic strains within mosquito individuals with a remarkable habitat-specificity. 
PY 2022
SO Computational And Structural Biotechnology Journal
SN 2001-0370
PU Elsevier BV
VL 20
UT 000819903300012
BP 410
EP 420
DI 10.1016/j.csbj.2021.12.019
ID 85332
ER

EF