FN Archimer Export Format
PT J
TI A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea
BT 
AF Remize, Marine
   Planchon, Frederic
   Garnier, Matthieu
   Loh, Ai Ning
   Le Grand, Fabienne
   Bideau, Antoine
   Lambert, Christophe
   Corvaisier, Rudolph
   Volety, Aswani
   Soudant, Philippe
AS 1:1,2;2:1;3:3;4:4;5:6;6:1;7:6;8:6;9:5;10:6;
FF 1:;2:;3:PDG-RBE-BRM-LPBA;4:;5:;6:;7:;8:;9:;10:;
C1 UMR 6539 LEMAR, CNRS, IRD, Ifremer, University of Brest, 29280 Plouzane, France
   GREENSEA, Promenade du Sergeant Navarro, 34140 Meze, France
   PBA, Ifremer, Rue de l’Ile d’Yeu, BP 21105, CEDEX 03, 44311 Nantes, France
   Center for Marine Science, Department of Earth and Ocean Sciences, University of North Carolina Wilmington, 5600 Marvin K. Moss Ln, Wilmington, NC 28403, USA
   50 Campus Drive, Elon University, Elon, NC 27244, USA
   UMR 6539 LEMAR, CNRS, IRD, Ifremer, University of Brest, 29280 Plouzane, France
C2 UBO, FRANCE
   GREENSEA, FRANCE
   IFREMER, FRANCE
   UNIV N CAROLINA, USA
   UNIV ELON, USA
   CNRS, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 5.4
TC 3
UR https://archimer.ifremer.fr/doc/00742/85353/90408.pdf
   https://archimer.ifremer.fr/doc/00742/85353/90410.zip
LA English
DT Article
DE ;long-chain PUFA synthesis;desaturases;elongases;PKS pathway;20;5n-3 (EPA);22;6n-3 (DHA);Tisochrysis lutea;C-13 artificial enrichment
AB The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a 13C-enriched isotopic marker, 13CO2, for 24 h during the exponential growth of the algae. The 13C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in 13C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in 13C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the 13C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis. 
PY 2022
PD JAN
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 20
IS 1
UT 000757562600001
DI 10.3390/md20010022
ID 85353
ER

EF