FN Archimer Export Format
PT J
TI Glycosaminoglycan-like sulfated polysaccharides from Vibrio diabolicus bacterium: Semi-synthesis and characterization
BT 
AF Esposito, Fabiana
   Vessella, Giulia
   Sinquin, Corinne
   Traboni, Serena
   Iadonisi, Alfonso
   Colliec-Jouault, Sylvia
   Zykwinska, Agata
   Bedini, Emiliano
AS 1:1;2:1;3:2;4:1;5:1;6:2;7:2;8:1;
FF 1:;2:;3:PDG-RBE-BRM-LEMMMB;4:;5:;6:PDG-RBE-BRM-LEMMMB;7:PDG-RBE-BRM-LEMMMB;8:;
C1 Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S.Angelo, via Cintia 4, I-80126 Napoli, Italy
   IFREMER, BRM, 44300 Nantes, France
C2 UNIV NAPLES, ITALY
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer UPR
   copubli-europe
IF 11.2
TC 10
UR https://archimer.ifremer.fr/doc/00742/85410/90471.pdf
LA English
DT Article
DE ;Exopolysaccharides;Glycosaminoglycans;Semi-synthesis;Sulfation;Growth factors
AB Sulfated glycosaminoglycan (GAG) analogues derived from plant, algae or microbial sourced polysaccharides are highly interesting in order to gain bioactivities similar to sulfated GAGs but without risks and concerns derived from their typical animal sources. Since the exopolysaccharide (EPS) produced by the bacterium Vibrio diabolicus HE800 strain from deep-sea hydrothermal vents is known to have a GAG-like structure with a linear backbone composed of unsulfated aminosugar and uronic acid monomers, its structural modification through four different semi-synthetic sulfation strategies has been performed. A detailed structural characterization of the six obtained polysaccharides revealed that three different sulfation patterns (per-O-sulfation, a single N-sulfation and a selective primary hydroxyls sulfation) were achieved, with molecular weights ranging from 5 to 40 kDa. A Surface Plasmonic Resonance (SPR) investigation of the affinity between such polysaccharides and a set of growth factors revealed that binding strength is primarily depending on polysaccharide sulfation degree. 
PY 2022
PD MAY
SO Carbohydrate Polymers
SN 0144-8617
PU Elsevier BV
VL 283
UT 000761002900002
DI 10.1016/j.carbpol.2021.119054
ID 85410
ER

EF