The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling

Type Article
Date 2022-01
Language English
Author(s) Scarsini Matteo1, Thiriet-Rupert Stanislas1, 2, Veidl Brigitte1, Mondeguer FlorenceORCID3, Hu Hanhua4, Marchand Justine1, Schoefs Benoît1
Affiliation(s) 1 : Metabolism, Bio-Engineering of Microalgal Molecules and Applications (MIMMA), Mer Molécules Santé, IUML—FR 3473 CNRS, Le Mans University, Le Mans, France
2 : Institut Pasteur, Genetics of Biofilms Laboratory, Paris, France
3 : Phycotoxins Laboratory, Institut Français de Recherche pour l'Exploitation de la Mer, Nantes, France
4 : Key Laboratory of Algal Biology, Chinese Academy of Sciences, Wuhan, China
Source Frontiers In Plant Science (1664-462X) (Frontiers Media SA), 2022-01 , Vol. 12 , P. 760516 (19p.)
DOI 10.3389/fpls.2021.760516
WOS© Times Cited 2
Keyword(s) carbon metabolism reorientation, stress, biotechnology, lipids, transcriptomics, turbidostat operated photobioreactor, pigments, photosynthesis

Microalgae have adapted to face abiotic stresses by accumulating energy storage molecules such as lipids, which are also of interest to industries. Unfortunately, the impairment in cell division during the accumulation of these molecules constitutes a major bottleneck for the development of efficient microalgae-based biotechnology processes. To address the bottleneck, a multidisciplinary approach was used to study the mechanisms involved in the transition from nitrogen repletion to nitrogen starvation conditions in the marine diatom Phaeodactylum tricornutum that was cultured in a turbidostat. Combining data demonstrate that the different steps of nitrogen deficiency clustered together in a single state in which cells are in equilibrium with their environment. The switch between the nitrogen-replete and the nitrogen-deficient equilibrium is driven by intracellular nitrogen availability. The switch induces a major gene expression change, which is reflected in the reorientation of the carbon metabolism toward an energy storage mode while still operating as a metabolic flywheel. Although the photosynthetic activity is reduced, the chloroplast is kept in a stand-by mode allowing a fast resuming upon nitrogen repletion. Altogether, these results contribute to the understanding of the intricate response of diatoms under stress.

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Scarsini Matteo, Thiriet-Rupert Stanislas, Veidl Brigitte, Mondeguer Florence, Hu Hanhua, Marchand Justine, Schoefs Benoît (2022). The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling. Frontiers In Plant Science, 12, 760516 (19p.). Publisher's official version : , Open Access version :