FN Archimer Export Format
PT J
TI The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling
BT 
AF Scarsini, Matteo
   Thiriet-Rupert, Stanislas
   Veidl, Brigitte
   Mondeguer, Florence
   Hu, Hanhua
   Marchand, Justine
   Schoefs, Benoît
AS 1:1;2:1,2;3:1;4:3;5:4;6:1;7:1;
FF 1:;2:;3:;4:PDG-ODE-PHYTOX;5:;6:;7:;
C1 Metabolism, Bio-Engineering of Microalgal Molecules and Applications (MIMMA), Mer Molécules Santé, IUML—FR 3473 CNRS, Le Mans University, Le Mans, France
   Institut Pasteur, Genetics of Biofilms Laboratory, Paris, France
   Phycotoxins Laboratory, Institut Français de Recherche pour l'Exploitation de la Mer, Nantes, France
   Key Laboratory of Algal Biology, Chinese Academy of Sciences, Wuhan, China
C2 UNIV LE MANS, FRANCE
   INST PASTEUR, FRANCE
   IFREMER, FRANCE
   CHINESE ACAD SCI, CHINA
SI NANTES
SE PDG-ODE-PHYTOX
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 5.6
TC 8
UR https://archimer.ifremer.fr/doc/00746/85811/90959.pdf
   https://archimer.ifremer.fr/doc/00746/85811/90960.docx
LA English
DT Article
DE ;carbon metabolism reorientation;stress;biotechnology;lipids;transcriptomics;turbidostat operated photobioreactor;pigments;photosynthesis
AB Microalgae have adapted to face abiotic stresses by accumulating energy storage molecules such as lipids, which are also of interest to industries. Unfortunately, the impairment in cell division during the accumulation of these molecules constitutes a major bottleneck for the development of efficient microalgae-based biotechnology processes. To address the bottleneck, a multidisciplinary approach was used to study the mechanisms involved in the transition from nitrogen repletion to nitrogen starvation conditions in the marine diatom Phaeodactylum tricornutum that was cultured in a turbidostat. Combining data demonstrate that the different steps of nitrogen deficiency clustered together in a single state in which cells are in equilibrium with their environment. The switch between the nitrogen-replete and the nitrogen-deficient equilibrium is driven by intracellular nitrogen availability. The switch induces a major gene expression change, which is reflected in the reorientation of the carbon metabolism toward an energy storage mode while still operating as a metabolic flywheel. Although the photosynthetic activity is reduced, the chloroplast is kept in a stand-by mode allowing a fast resuming upon nitrogen repletion. Altogether, these results contribute to the understanding of the intricate response of diatoms under stress. 
PY 2022
PD JAN
SO Frontiers In Plant Science
SN 1664-462X
PU Frontiers Media SA
VL 12
UT 000750682600001
DI 10.3389/fpls.2021.760516
ID 85811
ER

EF