FN Archimer Export Format
PT J
TI Amorphous-to-crystal transition in the layer-by-layer growth of bivalve shell prisms
BT 
AF Duboisset, Julien
   Ferrand, Patrick
   Baroni, Arthur
   Grünewald, Tilman A.
   Dicko, Hamadou
   Grauby, Olivier
   Vidal-Dupiol, Jeremie
   SAULNIER, Denis
   LE MOULLAC, Gilles
   Rosenthal, Martin
   Burghammer, Manfred
   Nouet, Julius
   Chevallard, Corinne
   Baronnet, Alain
   Chamard, Virginie
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:4;9:4;10:5;11:5;12:6;13:7;14:2;15:1;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-IHPE;8:PDG-RBE-RMPF;9:PDG-RBE-RMPF;10:;11:;12:;13:;14:;15:;
C1 Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
   Aix-Marseille Univ, CNRS, CINaM, Campus Luminy, Case 913, 13288-Marseille cedex 9, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier France
   Ifremer, UMR 241 Environnement Insulaire Océanien (EIO), Labex Corail, Centre du Pacifique, BP 49, Vairao 98719, French Polynesia
   European Synchrotron Radiation Facility, F-38043 Grenoble Cedex, France
   GEOPS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, 91405 Orsay, France
   NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette Cedex, France
C2 UNIV AIX MARSEILLE, FRANCE
   UNIV AIX MARSEILLE, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   ESRF, FRANCE
   UNIV PARIS SACLAY, FRANCE
   UNIV PARIS SACLAY, FRANCE
SI MONTPELLIER
   TAHITI
SE PDG-RBE-IHPE
   PDG-RBE-RMPF
UM IHPE
   EIO
IN WOS Ifremer UMR
   copubli-france
   copubli-univ-france
IF 9.7
TC 7
UR https://archimer.ifremer.fr/doc/00747/85863/91062.pdf
   https://archimer.ifremer.fr/doc/00747/85863/91063.docx
LA English
DT Article
DE ;Biomineralization;Mollusk prisms;Pinctada margaritifera;Pinna nobilis;Coherent raman microscopy;Vectorial ptychography
AB Biomineralization integrates complex physical and chemical processes bio-controlled by the living organisms through ionic concentration regulation and organic molecules production. It allows tuning the structural, optical and mechanical properties of hard tissues during ambient-condition crystallisation, motivating a deeper understanding of the underlying processes. By combining state-of-the-art optical and X-ray microscopy methods, we investigated early-mineralized calcareous units from two bivalve species, Pinctada margaritifera and Pinna nobilis, revealing chemical and crystallographic structural insights. In these calcite units, we observed ring-like structural features correlated with a lack of calcite and an increase of amorphous calcium carbonate and proteins contents. The rings also correspond to a larger crystalline disorder and a larger strain level. Based on these observations, we propose a temporal biomineralization cycle, initiated by the production of an amorphous precursor layer, which further crystallizes with a transition front progressing radially from the unit center, while the organics are expelled towards the prism edge. Simultaneously, along the shell thickness, the growth occurs following a layer-by-layer mode. These findings open biomimetic perspectives for the design of refined crystalline materials. Statement of Significance Calcareous biominerals are among the most present forms of biominerals. They exhibit astonishing structural, optical and mechanical properties while being formed at ambient synthesis conditions from ubiquitous ions, motivating the deep understanding of biomineralization. Here, we unveil the first formation steps involved in the biomineralization cycle of prismatic units of two bivalve species by applying a new multi-modal non-destructive characterization approach, sensitive to chemical and crystalline properties. The observations of structural features in mineralized units of different ages allowed the derivation of a temporal sequence for prism biomineralization, involving an amorphous precursor, a radial crystallisation front and a layer-by-layer sequence. Beyond these chemical and physical findings, the herein introduced multi-modal approach is highly relevant to other biominerals and bio-inspired studies. 
PY 2022
PD APR
SO Acta Biomaterialia
SN 1742-7061
PU Elsevier BV
VL 142
UT 000804657200006
BP 194
EP 207
DI 10.1016/j.actbio.2022.01.024
ID 85863
ER

EF