Balanced Polymorphism at the Pgm-1 Locus of the Pompeii Worm Alvinella pompejana and Its Variant Adaptability Is Only Governed by Two QE Mutations at Linked Sites

Type Article
Date 2022-02
Language English
Author(s) Bioy Alexis1, Le Port Anne-Sophie1, Sabourin Emeline2, Verheye Marie3, Piccino PatriceORCID4, Faure Baptiste5, Hourdez Stéphane6, Mary Jean1, Jollivet DidierORCID1
Affiliation(s) 1 : Adaptation et Diversité en Milieu Marin, Equipe ABICE, Station Biologique de Roscoff, UMR 7144 Sorbonne Université-CNRS, 29688 Roscoff, France
2 : Institut de Recherche Pour la Conservation des Zones Humides Méditerranéennes, Tour du Varlat, Le Sambuc, 13200 Arles, France
3 : Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000 Brussels, Belgium
4 : Lycée Lakanal, 3 Avenue du Président Franklin Roosevelt, 92330 Sceaux, France
5 : Biotope—Agence Nord-Littoral, ZA de la Maie, Avenue de l’Europe, 62720 Rinxent, France
6 : Observatoire Oceanologique de Banyuls-sur-Mer, UMR 8222 CNRS-SU, 1 Avenue Pierre Fabre, 66650 Banyuls-sur-Mer, France
Source Genes (2073-4425) (MDPI AG), 2022-02 , Vol. 13 , N. 2 , P. 206 (25p.)
DOI 10.3390/genes13020206
WOS© Times Cited 1
Note This article belongs to the Section Population and Evolutionary Genetics and Genomics
Keyword(s) phosphoglucomutase, balancing selection, thermal stability, gene, adaptive mutations, Alvinellidae
Abstract

The polychaete Alvinella pompejana lives exclusively on the walls of deep-sea hydrothermal chimneys along the East Pacific Rise (EPR), and displays specific adaptations to withstand the high temperatures and hypoxia associated with this highly variable habitat. Previous studies have revealed the existence of a balanced polymorphism on the enzyme phosphoglucomutase associated with thermal variations, where allozymes 90 and 100 exhibit different optimal activities and thermostabilities. Exploration of the mutational landscape of phosphoglucomutase 1 revealed the maintenance of four highly divergent allelic lineages encoding the three most frequent electromorphs over the geographic range of A. pompejana. This polymorphism is only governed by two linked amino acid replacements, located in exon 3 (E155Q and E190Q). A two-niche model of selection, including ‘cold’ and ‘hot’ conditions, represents the most likely scenario for the long-term persistence of these isoforms. Using directed mutagenesis and the expression of the three recombinant variants allowed us to test the additive effect of these two mutations on the biochemical properties of this enzyme. Our results are coherent with those previously obtained from native proteins, and reveal a thermodynamic trade-off between protein thermostability and catalysis, which is likely to have maintained these functional phenotypes prior to the geographic separation of populations across the Equator about 1.2 million years ago

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Bioy Alexis, Le Port Anne-Sophie, Sabourin Emeline, Verheye Marie, Piccino Patrice, Faure Baptiste, Hourdez Stéphane, Mary Jean, Jollivet Didier (2022). Balanced Polymorphism at the Pgm-1 Locus of the Pompeii Worm Alvinella pompejana and Its Variant Adaptability Is Only Governed by Two QE Mutations at Linked Sites. Genes, 13(2), 206 (25p.). Publisher's official version : https://doi.org/10.3390/genes13020206 , Open Access version : https://archimer.ifremer.fr/doc/00748/86009/