FN Archimer Export Format
PT J
TI Balanced Polymorphism at the Pgm-1 Locus of the Pompeii Worm Alvinella pompejana and Its Variant Adaptability Is Only Governed by Two QE Mutations at Linked Sites
BT 
AF Bioy, Alexis
   Le Port, Anne-Sophie
   Sabourin, Emeline
   Verheye, Marie
   Piccino, Patrice
   Faure, Baptiste
   Hourdez, Stéphane
   Mary, Jean
   Jollivet, Didier
AS 1:1;2:1;3:2;4:3;5:4;6:5;7:6;8:1;9:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 Adaptation et Diversité en Milieu Marin, Equipe ABICE, Station Biologique de Roscoff, UMR 7144 Sorbonne Université-CNRS, 29688 Roscoff, France
   Institut de Recherche Pour la Conservation des Zones Humides Méditerranéennes, Tour du Varlat, Le Sambuc, 13200 Arles, France
   Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000 Brussels, Belgium
   Lycée Lakanal, 3 Avenue du Président Franklin Roosevelt, 92330 Sceaux, France
   Biotope—Agence Nord-Littoral, ZA de la Maie, Avenue de l’Europe, 62720 Rinxent, France
   Observatoire Oceanologique de Banyuls-sur-Mer, UMR 8222 CNRS-SU, 1 Avenue Pierre Fabre, 66650 Banyuls-sur-Mer, France
C2 CNRS, FRANCE
   TOUR VALAT, FRANCE
   ROYAL BELGIAN INST NAT SCI, BELGIUM
   LYCEE LAKANAL, FRANCE
   BIOTOPE HAUTS FRANCE, FRANCE
   CNRS, FRANCE
IN DOAJ
IF 3.5
TC 1
UR https://archimer.ifremer.fr/doc/00748/86009/91232.pdf
   https://archimer.ifremer.fr/doc/00748/86009/91233.zip
LA English
DT Article
CR BIOSPEEDO
   MESCAL_LEG1
   MESCAL_LEG2
   PHARE
BO L'Atalante
DE ;phosphoglucomutase;balancing selection;thermal stability;gene;adaptive mutations;Alvinellidae
AB The polychaete Alvinella pompejana lives exclusively on the walls of deep-sea hydrothermal chimneys along the East Pacific Rise (EPR), and displays specific adaptations to withstand the high temperatures and hypoxia associated with this highly variable habitat. Previous studies have revealed the existence of a balanced polymorphism on the enzyme phosphoglucomutase associated with thermal variations, where allozymes 90 and 100 exhibit different optimal activities and thermostabilities. Exploration of the mutational landscape of phosphoglucomutase 1 revealed the maintenance of four highly divergent allelic lineages encoding the three most frequent electromorphs over the geographic range of A. pompejana. This polymorphism is only governed by two linked amino acid replacements, located in exon 3 (E155Q and E190Q). A two-niche model of selection, including ‘cold’ and ‘hot’ conditions, represents the most likely scenario for the long-term persistence of these isoforms. Using directed mutagenesis and the expression of the three recombinant variants allowed us to test the additive effect of these two mutations on the biochemical properties of this enzyme. Our results are coherent with those previously obtained from native proteins, and reveal a thermodynamic trade-off between protein thermostability and catalysis, which is likely to have maintained these functional phenotypes prior to the geographic separation of populations across the Equator about 1.2 million years ago 
PY 2022
PD FEB
SO Genes
SN 2073-4425
PU MDPI AG
VL 13
IS 2
UT 000790598400001
DI 10.3390/genes13020206
ID 86009
ER

EF