FN Archimer Export Format
PT J
TI Investigating the Environmental Survival of Marteilia refringens, a Marine Protozoan Parasite of the Flat Oyster Ostrea edulis, Through an Environmental DNA and Microscopy-Based Approach
BT 
AF MEROU, Nicolas
   Lecadet, Cyrielle
   Billon, Tom
   Chollet, Bruno
   Pouvreau, Stephane
   Arzul, Isabelle
AS 1:1;2:1;3:1;4:1;5:2;6:1;
FF 1:PDG-RBE-SGMM-LGPMM;2:PDG-RBE-ASIM;3:;4:PDG-RBE-ASIM;5:PDG-RBE-PFOM-LPI;6:PDG-RBE-ASIM;
C1 Santé, Génétique et Microbiologie des Mollusques (SG2M)-Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Institut Français de Recherche pour l’Exploitation de la Mer (Ifremer), La Tremblade, France
   Laboratoire des Sciences de l’Environnement Marin, UMR 6539 Ifremer/Université de Bretagne Occidentale (UBO)/Institut de Recherche pour le Développement (IRD)/Centre National de la Recherche Scientifique (CNRS), Ifremer, Argenton-en-Landunvez, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   ARGENTON
SE PDG-RBE-SGMM-LGPMM
   PDG-RBE-ASIM
   PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UPR
   WOS Ifremer UMR
   DOAJ
IF 3.7
TC 2
UR https://archimer.ifremer.fr/doc/00748/86010/91235.pdf
   https://archimer.ifremer.fr/doc/00748/86010/91236.xls
LA English
DT Article
DE ;Marteilia refringens;Ostrea edulis;survival;protozoan;parasite;shellfish;eDNA;transmission electron microscopy
AB Outside-host parasite survival is a key parameter to better understand disease mechanisms, especially for marine pathogens transmitted from one host to another through an environmental stage. For non-cultivable micro-parasites like Marteilia refringens, a protozoan parasite infecting the flat oyster Ostrea edulis, investigating this parameter requires innovative approaches. In the present study, we have developed an Environmental DNA (eDNA)-based method allowing detecting and quantifying up to 25 and 10 parasites DNA in seawater and sediment, respectively. This method was used in combination with light and transmission electron microscopy (TEM) to study experimentally parasite survival in seawater and flat oyster faeces after its release from naturally infected oysters. M. refringens DNA could be detected up to 20 days, in both seawater and oyster faeces with a more stable detection over time in the latter. Light and transmission microscopy confirm that parasites leaving flat oysters are sporangia. We also observed a membrane dissolution of the sporangia over time that could indicate the release of parasite spore. This study not only improves our understanding of M. refringens ecology but also highlights the interest to combine molecular and microscopical analysis to study non-cultivable micro-parasites life cycle outside their host. 
PY 2022
PD JAN
SO Frontiers In Marine Science
SN 2296-7745
PU Frontiers Media SA
VL 9
UT 000756931100001
DI 10.3389/fmars.2022.811284
ID 86010
ER

EF