FN Archimer Export Format
PT J
TI Microgels based on Infernan, a glycosaminoglycan-mimetic bacterial exopolysaccharide, as BMP-2 delivery systems
BT 
AF Gélébart, Perrine
   Cuenot, Stéphane
   Sinquin, Corinne
   Halgand, Boris
   Sourice, Sophie
   Le Visage, Catherine
   Guicheux, Jérôme
   Colliec-Jouault, Sylvia
   Zykwinska, Agata
AS 1:1;2:2;3:1;4:3;5:3,4;6:3,4;7:3,4,5;8:1;9:1;
FF 1:PDG-RBE-BRM-LEMMMB;2:;3:PDG-RBE-MASAE-LEMMMB;4:;5:;6:;7:;8:PDG-RBE-MASAE-LEMMMB;9:PDG-RBE-MASAE-LEMMMB;
C1 Ifremer, MASAE, 44300 Nantes, France
   Nantes Université, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes, France
   Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, F-44042 Nantes, France
   UFR Odontologie, Université de Nantes, Nantes, France
   CHU Nantes, PHU4 OTONN, Nantes, France
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   INSERM, FRANCE
   UNIV NANTES, FRANCE
   CHU NANTES, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
   PDG-RBE-MASAE-LEMMMB
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 11.2
TC 7
UR https://archimer.ifremer.fr/doc/00750/86163/100001.pdf
LA English
DT Article
DE ;Heparin-mimetic;Capillary microfluidics;Release kinetics;BMP-2 bioactivity;Osteogenic potential;AFM imaging
AB Bone Morphogenetic Protein (BMP-2) is an osteoinductive growth factor clinically used for bone regeneration. Tuneable sustained strategies for BMP-2 delivery are intensely developed to avoid severe complications related to supraphysiological doses applied. To address this issue, we investigated the ability of the bacterial exopolysaccharide (EPS) called Infernan produced by the deep-sea hydrothermal vent bacterium Alteromonas infernus, exhibiting both glycosaminoglycan-mimetic and physical gelling properties, to efficiently bind and release the bioactive BMP-2. Two delivery systems were designed based on BMP-2 retention in either single or complex EPS-based microgels, both manufactured using a microfluidic approach. BMP-2 release kinetics were highly influenced by the ionic strength, affecting both microgel stability and growth factor/EPS binding, appearing essential for BMP-2 bioactivity. The osteogenic activity of human bone-marrow derived mesenchymal stem cells studied in vitro emphasized that Infernan microgels constitute a promising platform for BMP-2 delivery for further in vivo bone repair. 
PY 2022
PD MAY
SO Carbohydrate Polymers
SN 0144-8617
PU Elsevier BV
VL 284
UT 000761037700008
DI 10.1016/j.carbpol.2022.119191
ID 86163
ER

EF