FN Archimer Export Format
PT J
TI First insights on the diversity of the genus Ostreopsis (Dinophyceae, Gonyaulacales) in Guadeloupe Island, with emphasis on the phylogenetic position of O. heptagona
BT 
AF Boisnoir, Aurelie
   Bilien, Gwenael
   Lemée, Rodolphe
   Chomérat, Nicolas
AS 1:1;2:2;3:3;4:2;
FF 1:PDG-RBE-BIODIVENV;2:PDG-ODE-LITTORAL-LERBO;3:;4:PDG-ODE-LITTORAL-LERBO;
C1 Ifremer, BIODIVENV, F-97231 Le Robert, Martinique, France
   Ifremer, LITTORAL, F-29900 Concarneau, France
   Sorbonne Université, CNRS, Laboratoire d’Océanographie de Villefranche, Villefranche-sur-Mer, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV SORBONNE, FRANCE
SI MARTINIQUE
   CONCARNEAU
SE PDG-RBE-BIODIVENV
   PDG-ODE-LITTORAL-LERBO
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 2.9
TC 4
UR https://archimer.ifremer.fr/doc/00751/86257/91561.pdf
LA English
DT Article
DE ;Amoebophrya;Concatenated phylogeny;Morpho-molecular taxonomy;Ostreopsis cf;ovata;Ostreopsis lenticularis;Ostreopsis siamensis;Ribosomal operon
AB The present study aims to identify epiphytic Ostreopsis cells collected in Guadeloupe between 2017 and 2018 using a morpho-molecular approach. This method combined microscopical observations of wild specimens (light and scanning electron microscopy) with a phylogenetic analysis inferred from concatenated sequences of ribosomal operon (SSU + ITS + LSU) of Ostreopsidoideae. Four distinct morphotypes were identified in our samples and studied by SEM. Molecular data obtained from single-cell PCR for the four morphotypes were consistent with observations and confirmed the presence of three Ostreopsis species resolved in well characterized genotypes (O. cf. ovata, O. lenticularis and O. siamensis) and an unidentified clade. Detailed morphological characters including sulcal plates confirmed the identification of the last morphotype as O. heptagona D.R.Norris, J.W.Bomber & Balech, which forms a new basal clade in the genus, not previously reported. Observations highlighted overlapping sizes for O. lenticularis, O. siamensis and O. heptagona. Direct sequencing of PCR products obtained for some O. lenticularis and O. heptagona collected at one site revealed unexpectedly the presence of the parasitoid dinoflagellate Amoebophrya. Some Ostreopsis cells were found partially emptied and exhibiting a compact mass. Further analyses are needed to understand the ecological role of Amoebophrya on blooms of epiphytic Ostreopsis species. 
PY 2022
PD APR
SO European Journal Of Protistology
SN 0932-4739
PU Elsevier BV
VL 83
UT 000783949700005
DI 10.1016/j.ejop.2022.125875
ID 86257
ER

EF