FN Archimer Export Format PT J TI Trophic transfer of copper decreases the condition index in Crassostrea gigas spat in concomitance with a change in the microalgal fatty acid profile and enhanced oyster energy demand BT AF Akcha, Farida Coquillé, Nathalie Sussarellu, Rossana ROUXEL, Julien Chouvelon, Tiphaine Gonzalez, P. Legeay, A. Bruzac, Sandrine Sireau, Teddy Gonzalez, Jean-Louis Gourves, P.-Y. GODFRIN, Yoann Buchet, Vincent Stachowski-Haberkorn, Sabine AS 1:1;2:1;3:1;4:1;5:2;6:3;7:3;8:2;9:2;10:2;11:3;12:1;13:4;14:1; FF 1:PDG-RBE-CCEM;2:PDG-RBE-BE-LEX;3:PDG-ODE-PHYTOX-GENALG;4:;5:PDG-RBE-CCEM;6:;7:;8:PDG-RBE-CCEM;9:PDG-RBE-CCEM;10:PDG-ODE-LITTORAL-LERPAC;11:;12:PDG-RBE-CCEM;13:PDG-RBE-SGMM-PMMB;14:PDG-ODE-PHYTOX-PHYSALG; C1 Ifremer, Laboratory of Ecotoxicology, 44311 Nantes cedex 03, France Ifremer, Laboratory of Biogeochemistry of Metallic Contaminants, 44311 Nantes cedex 03, France University of Bordeaux, CNRS, EPOC, UMR 5805, Aquatic Ecotoxicology, Arcachon, France Ifremer, Experimental Facilities for Marine Mollusks, 85230 Bouin, France C2 IFREMER, FRANCE IFREMER, FRANCE UNIV BORDEAUX, FRANCE IFREMER, FRANCE SI NANTES TOULON BOUIN SE PDG-RBE-CCEM PDG-RBE-BE-LEX PDG-ODE-PHYTOX-GENALG PDG-ODE-LITTORAL-LERPAC PDG-RBE-SGMM-PMMB PDG-ODE-PHYTOX-PHYSALG IN WOS Ifremer UPR copubli-france copubli-univ-france IF 9.8 TC 5 UR https://archimer.ifremer.fr/doc/00751/86340/91656.pdf LA English DT Article DE ;Cu;Tetraselmis suecica;Oyster;Trophic exposure;Toxicity;Lipid composition AB Due to new usages and sources, copper (Cu) concentrations are increasing in the Arcachon Basin, an important shellfish production area in France. In the present paper, the trophic transfer of Cu was studied between a microalga, Tetraselmis suecica, and Crassostrea gigas (Pacific oyster) spat. An experimental approach was developed to assess Cu exposure, transfer and toxicity on both phytoplankton and spat. Exposure of microalgal cultures to Cu for 7–8 days (3.1 ± 0.1, 15.7 ± 0.2 and 50.4 ± 1.0 μg Cu·L−1 for the control, Cu15 and Cu50 conditions, respectively) led to concentrations in microalgae (28.3 ± 0.9 and 110.7 ± 11.9 mg Cu·kg dry weight−1 for Cu15 and Cu50, respectively) close to those measured in the field. Despite Cu accumulation, the physiology of the microalgae remained poorly affected. Exposed cultures could only be discriminated from controls by a higher relative content in intracellular reactive oxygen species, and a lower relative content in lipids together with a reduced metabolic activity. By contrast, the fatty acid profile of microalgae was modified, with a particularly relevant lower content of the essential polyunsaturated fatty acid 22:6n-3 (docosahexaenoic acid [DHA]). Following 21 days of spat feeding with Cu15 and Cu50 microalgal cultures, trophic transfer of Cu was observed with a high initial Cu concentration in spat tissues. No effect was observed on oxidative stress endpoints. Cu exposure was responsible for a decrease in the spat condition index, an outcome that could be related to an insufficient DHA supply and extra energy demand as suggested by the overexpression of genes involved in energy metabolism, ATP synthesis and glycogen catabolism. PY 2022 PD JUL SO Science Of The Total Environment SN 0048-9697 PU Elsevier BV VL 824 UT 000766161300067 DI 10.1016/j.scitotenv.2022.153841 ID 86340 ER EF