FN Archimer Export Format
PT J
TI The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis
BT 
AF Pajot, Anne
   Lavaud, Johann
   Carrier, Gregory
   Garnier, Matthieu
   Saint-Jean, Bruno
   Rabilloud, Noémie
   Baroukh, Caroline
   Bérard, Jean-Baptiste
   Bernard, Olivier
   Marchal, Luc
   Nicolau, Elodie
AS 1:1;2:2;3:1;4:1;5:1;6:1;7:1;8:1;9:3;10:4;11:1;
FF 1:PDG-ODE-PHYTOX-GENALG;2:;3:PDG-ODE-PHYTOX-GENALG;4:PDG-ODE-PHYTOX-GENALG;5:PDG-ODE-PHYTOX-GENALG;6:;7:;8:PDG-ODE-PHYTOX-PHYSALG;9:;10:;11:PDG-ODE-PHYTOX-GENALG;
C1 IFREMER, Physiology and Biotechnology of Algae Laboratory, Nantes, France
   LEMAR-Laboratoire des Sciences de l’Environnement Marin, UMR 6539, CNRS/Univ Brest/Ifremer/IRD, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Plouzané, France
   Université Côte d’Azur, Biocore, INRIA, CNRS, Sorbonne Université (LOV, UMR 7093), Sophia-Antipolis, France
   Univ Nantes, GEPEA, Nantes, France
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UNIV COTE D’AZUR, FRANCE
   UNIV NANTES, FRANCE
SI NANTES
SE PDG-ODE-PHYTOX-GENALG
   PDG-ODE-PHYTOX-PHYSALG
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 5.6
TC 8
UR https://archimer.ifremer.fr/doc/00752/86392/91707.pdf
   https://archimer.ifremer.fr/doc/00752/86392/91708.pdf
   https://archimer.ifremer.fr/doc/00752/86392/91709.xlsx
LA English
DT Article
DE ;carotenoids;fucoxanthin and chlorophyll a;c-binding proteins;Lhcf;Lhcr;Lhcx;Tisochrysis lutea;turbidostat culture;chemostat culture
AB We observed differences in lhc classification in Chromista. We proposed a classification of the lhcf family with two groups specific to haptophytes, one specific to diatoms, and one specific to seaweeds. Identification and characterization of the Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) of the haptophyte microalgae Tisochrysis lutea were performed by similarity analysis. The FCP family contains 52 lhc genes in T. lutea. FCP pigment binding site candidates were characterized on Lhcf protein monomers of T. lutea, which possesses at least nine chlorophylls and five fucoxanthin molecules, on average, per monomer. The expression of T. lutea lhc genes was assessed during turbidostat and chemostat experiments, one with constant light (CL) and changing nitrogen phases, the second with a 12 h:12 h sinusoidal photoperiod and changing nitrogen phases. RNA-seq analysis revealed a dynamic decrease in the expression of lhc genes with nitrogen depletion. We observed that T. lutea lhcx2 was only expressed at night, suggesting that its role is to protect \cells from return of light after prolonged darkness exposure. 
PY 2022
PD FEB
SO Frontiers In Plant Science
SN 1664-462X
PU Frontiers Media SA
VL 13
UT 000765069300001
DI 10.3389/fpls.2022.830069
ID 86392
ER

EF