FN Archimer Export Format PT J TI Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) BT AF Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique AS 1:1,2;2:3;3:4;4:5;5:6;6:5;7:1;8:7;9:5; FF 1:PDG-RBE-MASAE-LSEM;2:;3:;4:PDG-RBE-PFOM-LARN;5:PDG-RBE-EMMA-PMMB;6:PDG-RBE-PFOM-LPI;7:PDG-RBE-MASAE-LEMMMB;8:PDG-RBE-ASIM;9:PDG-RBE-PFOM-LARN; C1 Ifremer, MASAE, F‐44311 Nantes, France Ifremer, SGMM, F‐29280 Plouzané, France Nantes Université, CNRS, US2B, UMR6286 F‐44000 Nantes, France Univ Brest, CNRS, Ifremer, IRD, LEMAR, F‐29280 Plouzané, France Univ Brest, Ifremer, CNRS, IRD, LEMAR, F‐29280 Plouzané, France Ifremer, EMMA, F‐85230 Bouin, France Ifremer, ASIM, F‐17390 La Tremblade, France C2 IFREMER, FRANCE IFREMER, FRANCE UNIV NANTES, FRANCE CNRS, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE SI BREST BOUIN ARGENTON NANTES LA TREMBLADE SE PDG-RBE-MASAE-LSEM PDG-RBE-PFOM-LARN PDG-RBE-EMMA-PMMB PDG-RBE-PFOM-LPI PDG-RBE-MASAE-LEMMMB PDG-RBE-ASIM UM LEMAR IN WOS Ifremer UPR WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-univ-france IF 5.1 TC 3 UR https://archimer.ifremer.fr/doc/00763/87507/93012.tif https://archimer.ifremer.fr/doc/00763/87507/96196.pdf LA English DT Article AB Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Crassostrea gigas, we developed a GFP-tagged V. parahaemolyticus strain (IFVp201-gfp+), as well as a rapid and efficient quantification method in C. gigas oysters haemolymph by flow cytometry. Impact of the life-history of C. gigas on accumulation and depuration of V. parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V. parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates. PY 2022 PD SEP SO Environmental Microbiology SN 1462-2912 PU Wiley VL 24 IS 9 UT 000792510100001 BP 4401 EP 4410 DI 10.1111/1462-2920.15996 ID 87507 ER EF