FN Archimer Export Format
PT J
TI ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations
BT 
AF Rosani, Umberto
   Bortoletto, Enrico
   Montagnani, Caroline
   Venier, Paola
AS 1:1;2:1;3:2;4:1;
FF 1:;2:;3:PDG-RBE-IHPE;4:;
C1 Department of Biology, University of Padova, Padova, Italy
   IHPE, CNRS, Ifremer, Université Montpellier, Montpellier, France
C2 UNIV PADOVA, ITALY
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer UMR
   DOAJ
   copubli-europe
IF 4.8
TC 3
UR https://archimer.ifremer.fr/doc/00764/87629/93063.pdf
   https://archimer.ifremer.fr/doc/00764/87629/93064.docx
   https://archimer.ifremer.fr/doc/00764/87629/93065.docx
   https://archimer.ifremer.fr/doc/00764/87629/93066.docx
   https://archimer.ifremer.fr/doc/00764/87629/93067.docx
   https://archimer.ifremer.fr/doc/00764/87629/93068.xlsx
LA English
DT Article
DE ;ADAR1;hyper-editing;oyster;OsHV-1;antiviral immunity;ADAR;RNA editing;bivalve;innate immunity;malacoherpesvirus
AB Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq data sets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ∼0.5% of the oyster transcriptome and including several tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation, and epigenetic regulation that were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve “RNA editome,” available tools and technologies can be adapted to bivalve mollusks. IMPORTANCE Ostreid herpesvirus-1 (OsHV-1) is a harmful pathogen of bivalve species, such as oysters. However, knowledge is lacking about host–virus interactions at the molecular level, hampering the possibility of a correct management of viral outbreaks and related massive mortalities. Notably, OsHV-1 transcripts are massively modified by host RNA editing enzyme during infection, resulting in multiple A-to-I variations along RNAs assuming double-strand conformations. The impact of these modifications on host transcripts is, however, not completely clear. Analyzing RNA-seq data of oysters infected with OsHV-1, we revealed that ∼0.5% of the oyster transcriptome is always enzymatically modified by ADAR, whereas genes involved in antiviral response, miRNA maturation, and epigenetic regulation were hyper-edited in specific conditions only. Despite our results, relevant technical bottlenecks impair an accurate quantification of RNA editing events, making necessary an approach specifically dedicated to the progressive understanding of oyster “RNA editome.” 
PY 2022
PD APR
SO Msphere
SN 2379-5042
PU American Society for Microbiology
VL 7
IS 2
UT 000778021300001
DI 10.1128/msphere.00011-22
ID 87629
ER

EF