FN Archimer Export Format
PT J
TI Influence of the Carbon and Nitrogen Sources on Diabolican Production by the Marine Vibrio diabolicus Strain CNCM I-1629
BT 
AF DELBARRE LADRAT, Christine
   SINQUIN, Corinne
   MARCHAND, Laetitia
   BONNETOT, Sandrine
   ZYKWINSKA, Agata
   VERREZ-BAGNIS, Veronique
   COLLIEC JOUAULT, Sylvia
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;
FF 1:PDG-RBE-MASAE-LEMMMB;2:PDG-RBE-MASAE-LEMMMB;3:PDG-RBE-MASAE-LEMMMB;4:PDG-RBE-MASAE-LEMMMB;5:PDG-RBE-MASAE-LEMMMB;6:PDG-RBE-MASAE-LEMMMB;7:PDG-RBE-MASAE-LEMMMB;
C1 Ifremer, MASAE, EM3B Laboratory, CEDEX 3, F-44300 Nantes, France
C2 IFREMER, FRANCE
SI NANTES
SE PDG-RBE-MASAE-LEMMMB
IN WOS Ifremer UPR
   DOAJ
IF 5
TC 7
UR https://archimer.ifremer.fr/doc/00770/88227/93771.pdf
   https://archimer.ifremer.fr/doc/00770/88227/93790.zip
LA English
DT Article
DE ;exopolysaccharide;marine bacterium;production;yield;central composite design;molecular weight
AB Recent advances in glycobiotechnology show that bacterial exopolysaccharides (EPS) presenting glycosaminoglycan (GAG)-like properties can provide a valuable source of bio-active macromolecules for industrial applications. The HE800 EPS, named diabolican, is a marine-derived anionic high-molecular-weight polysaccharide produced by Vibrio diabolicus CNCM I-1629 which displays original structural features close to those of hyaluronic acid. We investigated the impact of carbon and nitrogen substrates on both Vibrio diabolicus growth and diabolican production. Both substrates were screened by a one-factor-at-a-time method, and experimental designs were used to study the effect of glucose, mannitol, and ammonium acetate various concentrations. Results showed that the medium composition affected not only the bacterium growth and EPS yield, but also the EPS molecular weight (MW). EPS yields of 563 and 330 mg L−1 were obtained in the presence of 69.3 g L−1 glucose and 24.6 g L−1 mannitol, respectively, both for 116.6 mM ammonium acetate. MW was the highest, with 69.3 g L−1 glucose and 101.9 mM ammonium acetate (2.3 × 106 g mol−1). In parallel, the bacterial maximum specific growth rate was higher when both carbon and nitrogen substrate concentrations were low. This work paves the way for the optimization of marine exopolysaccharide production of great interest in the fields of human health and cosmetics 
PY 2022
PD MAY
SO Polymers
SN 2073-4360
PU MDPI
VL 14
IS 10
UT 000801355700001
DI 10.3390/polym14101994
ID 88227
ER

EF