FN Archimer Export Format
PT J
TI Wide Distribution and Specific Resistance Pattern to Third-Generation Cephalosporins of Enterobacter cloacae Complex Members in Humans and in the Environment in Guadeloupe (French West Indies)
BT 
AF POT, Matthieu
   REYNAUD, Yann
   COUVIN, David
   DUCAT, Celia
   FERDINAND, Severine
   GRAVEY, Francois
   GRUEL, Gaelle
   GUERIN, Francois
   MALPOTE, Edith
   BREUREC, Sebastien
   TALARMIN, Antoine
   GUYOMARD-RABENIRINA, Stephanie
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:1;8:2,3;9:4;10:1,5,6;11:1;12:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Pasteur Inst Guadeloupe, Transmiss Reservoir & Divers Pathogens Unit, Les Abymes, France.
   Normandie Univ, UNIROUEN, UNICAEN, GRAM 2 0, Caen, France.
   Caen Univ Hosp, Dept Clin Microbiol, Caen, France.
   Univ Hosp Guadeloupe, Lab Clin Microbiol, Pointe A Pitre, Guadeloupe, France.
   Univ Antilles, Fac Med Hyacinthe Bastaraud, Pointe A Pitre, Guadeloupe, France.
   INSERM, Ctr Clin Invest 1424, Pointe A Pitre, Guadeloupe, France.
C2 INST PASTEUR GUADELOUPE, FRANCE
   UNIV NORMANDIE, FRANCE
   CAEN UNIV HOSP, FRANCE
   UNIV HOSP GUADELOUPE, FRANCE
   UNIV ANTILLES, FRANCE
   INSERM, FRANCE
IN DOAJ
IF 6.064
TC 12
UR https://archimer.ifremer.fr/doc/00771/88277/93848.pdf
   https://archimer.ifremer.fr/doc/00771/88277/93849.pdf
   https://archimer.ifremer.fr/doc/00771/88277/93850.xlsx
   https://archimer.ifremer.fr/doc/00771/88277/93851.xlsx
   https://archimer.ifremer.fr/doc/00771/88277/93852.xlsx
   https://archimer.ifremer.fr/doc/00771/88277/93853.xlsx
LA English
DT Article
DE ;Anolis marmoratus;cephalosporinase overproduction;Enterobacter cloacae complex;ESBL;hsp60;one health;phylogeny;Caribbean
AB Species belonging to Enterobacter cloacae complex have been isolated in numerous environments and samples of various origins. They are also involved in opportunistic infections in plants, animals, and humans. Previous prospection in Guadeloupe (French West Indies) indicated a high frequency of E. cloacae complex strains resistant to third-generation cephalosporins (3GCs) in a local lizard population (Anolis marmoratus), but knowledge of the distribution and resistance of these strains in humans and the environment is limited. The aim of this study was to compare the distribution and antibiotic susceptibility pattern of E. cloacae complex members from different sources in a "one health" approach and to find possible explanations for the high level of resistance in non-human samples. E. cloacae complex strains were collected between January 2017 and the end of 2018 from anoles, farm animals, local fresh produce, water, and clinical human samples. Isolates were characterized by the heat-shock protein 60 gene-fragment typing method, and whole-genome sequencing was conducted on the most frequent clusters (i.e., C-VI and C-VIII). The prevalence of resistance to 3GCs was relatively high (56/346, 16.2%) in non-human samples. The associated resistance mechanism was related to an AmpC overproduction; however, in human samples, most of the resistant strains (40/62) produced an extended-spectrum beta-lactamase. No relation was found between resistance in isolates from wild anoles (35/168) and human activities. Specific core-genome phylogenetic analysis highlighted an important diversity in this bacterial population and no wide circulation among the different compartments. In our setting, the mutations responsible for resistance to 3GCs, especially in ampD, were diverse and not compartment specific. In conclusion, high levels of resistance in non-human E. cloacae complex isolates are probably due to environmental factors that favor the selection of these resistant strains, and this will be explored further.
PY 2021
PD JUL
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 12
UT 000671395900001
DI 10.3389/fmicb.2021.628058
ID 88277
ER

EF