FN Archimer Export Format
PT J
TI Heterogeneity among Mycobacterium ulcerans from French Guiana Revealed by Multilocus Variable Number Tandem Repeat Analysis (MLVA)
BT 
AF REYNAUD, Yann
   MILLET, Julie
   COUVIN, David
   RASTOGI, Nalin
   BROWN, Christopher
   COUPPIE, Pierre
   LEGRAND, Eric
AS 1:1;2:2;3:2;4:2;5:3;6:4;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Inst Pasteur, Cayenne, French Guiana.
   Inst Pasteur Guadeloupe, Pointe A Pitre, Guadeloupe, France.
   NOAA, Milford, CT USA.
   Ctr Hosp Andre Rosemon, Cayenne, French Guiana.
C2 INST PASTEUR, FRANCE
   INST PASTEUR GUADELOUPE, FRANCE
   NOAA, USA
   CHU CAYENNE, FRANCE
IN DOAJ
IF 3.057
TC 6
UR https://archimer.ifremer.fr/doc/00771/88278/93927.pdf
LA English
DT Article
AB Buruli ulcer is an emerging and neglected tropical disease caused by Mycobacterium ulcerans. Few cases have been reported so far in the Americas. With 250 cases reported since 1969, French Guiana is the only Buruli ulcer endemic area in the continent. Thus far, no genetic diversity studies of strains of M. ulcerans from French Guiana have been reported. Our goal in the present study was to examine the genetic diversity of M. ulcerans strains in this region by using the Multilocus Variable Number Tandem Repeat Analysis (MLVA) approach. A total of 23 DNA samples were purified from ulcer biopsies or derived from pure cultures. MVLA was used in the study of six previously-described Variable Number of Tandem Repeat (VNTR) markers. A total of three allelic combinations were characterized in our study: genotype I which has been described previously, genotype III which is very similar to genotype I, and genotype II which has distinctly different characteristics in comparison with the other two genotypes. This high degree of genetic diversity appears to be uncommon for M. ulcerans. Further research based on complete genome sequencing of strains belonging to genotypes I and II is in progress and should lead soon to a better understanding of genetic specificities of M. ulcerans strains from French Guiana.
PY 2015
PD FEB
SO Plos One
SN 1932-6203
PU Public Library Science
VL 10
IS 2
UT 000350662100193
DI 10.1371/journal.pone.0118597
ID 88278
ER

EF