FN Archimer Export Format
PT J
TI Bayesian population structure analysis reveals presence of phylogeographically specific sublineages within previously ill-defined T group of Mycobacterium tuberculosis
BT 
AF REYNAUD, Yann
   ZHENG, Chao
   WU, Guihui
   SUN, Qun
   RASTOGI, Nalin
AS 1:1;2:1,2;3:3;4:2;5:1;
FF 1:;2:;3:;4:;5:;
C1 Inst Pasteur Guadeloupe, TB & Mycobacteria Unit, WHO Supranat TB Reference Lab, Morne Joliviere Abymes, Guadeloupe, France.
   Sichuan Univ, Coll Life Sci, Chengdu, Sichuan, Peoples R China.
   Chengdu Publ Hlth Clin Ctr, Chengdu, Sichuan, Peoples R China.
C2 INST PASTEUR GUADELOUPE, FRANCE
   UNIV SICHUAN, CHINA
   CHENGDU PUBL HLTH CLIN CTR, CHINA
IN DOAJ
IF 2.766
TC 5
UR https://archimer.ifremer.fr/doc/00771/88280/93904.pdf
   https://archimer.ifremer.fr/doc/00771/88280/93905.tif
   https://archimer.ifremer.fr/doc/00771/88280/93906.tif
   https://archimer.ifremer.fr/doc/00771/88280/93907.tif
   https://archimer.ifremer.fr/doc/00771/88280/93908.tif
   https://archimer.ifremer.fr/doc/00771/88280/93909.pot
   https://archimer.ifremer.fr/doc/00771/88280/93910.xls
   https://archimer.ifremer.fr/doc/00771/88280/93911.xlsx
   https://archimer.ifremer.fr/doc/00771/88280/93912.xlsx
LA English
DT Article
AB Mycobacterium tuberculosis genetic structure, and evolutionary history have been studied for years by several genotyping approaches, but delineation of a few sublineages remains controversial and needs better characterization. This is particularly the case of T group within lineage 4 (L4) which was first described using spoligotyping to pool together a number of strains with ill-defined signatures. Although T strains were not traditionally considered as a real phylogenetic group, they did contain a few phylogenetically meaningful sublineages as shown using SNPs. We therefore decided to investigate if this observation could be corroborated using other robust genetic markers. We consequently made a first assessment of genetic structure using 24-loci MIRU-VNTRs data extracted from the SITVIT2 database (n = 607 clinical isolates collected in Russia, Albania, Turkey, Iraq, Brazil and China). Combining Minimum Spanning Trees and Bayesian population structure analyses (using STRUCTURE and TESS softwares), we distinctly identified eight tentative phylogenetic groups (T1-T8) with a remarkable correlation with geographical origin. We further compared the present structure observed with other L4 sublineages (n = 416 clinical isolates belonging to LAM, Haarlem, X, S sublineages), and showed that 5 out of 8 T groups seemed phylogeographically well-defined as opposed to the remaining 3 groups that partially mixed with other L4 isolates. These results provide with novel evidence about phylogeographically specificity of a proportion of ill-defined T group of M. tuberculosis. The genetic structure observed will now be further validated on an enlarged worldwide dataset using Whole Genome Sequencing (WGS).
PY 2017
PD FEB
SO Plos One
SN 1932-6203
PU Public Library Science
VL 12
IS 2
UT 000393700100055
DI 10.1371/journal.pone.0171584
ID 88280
ER

EF