FN Archimer Export Format
PT J
TI Interactions between infernan and calcium: From the molecular level to the mechanical properties of microgels
BT 
AF Zykwinska, Agata
   Makshakova, Olga
   Gélébart, Perrine
   Sinquin, Corinne
   Stephant, Nicolas
   Colliec-Jouault, Sylvia
   Perez, Stella
   Cuenot, Stéphane
AS 1:1;2:2;3:1;4:1;5:3;6:1;7:4;8:3;
FF 1:PDG-RBE-MASAE-LEMMMB;2:;3:PDG-RBE-BRM-LEMMMB;4:PDG-RBE-MASAE-LEMMMB;5:;6:PDG-RBE-MASAE-LEMMMB;7:;8:;
C1 Ifremer, MASAE, 44300 Nantes, France
   Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, RAS, Lobachevsky Str., 2/31, 420111 Kazan, Russian Federation
   Nantes Université, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes, France
   Centre de Recherches sur les Macromolécules Végétales, Université de Grenoble Alpes, Centre National de la Recherche Scientifique, Grenoble, France
C2 IFREMER, FRANCE
   RUSSIAN ACAD SCI, RUSSIA
   UNIV NANTES, FRANCE
   CNRS, FRANCE
SI NANTES
SE PDG-RBE-MASAE-LEMMMB
   PDG-RBE-BRM-LEMMMB
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 11.2
TC 2
UR https://archimer.ifremer.fr/doc/00772/88367/93994.pdf
LA English
DT Article
DE ;Atomic force microscopy;Molecular dynamics simulations;Persistence length;Viscoelastic properties;Microfluidics
AB With the increasing need for hydrogels with tunable properties for specific biomedical applications, a complete understanding of the structure-function relationship of polymers used for hydrogel development remains crucial for their optimal use. In the present study, by combining experimental and theoretical approaches, the structure-function relationship of a bacterial exopolysaccharide, infernan, displaying both glycosaminoglycan-mimetic and gelling properties, was investigated at molecular and microscopic levels. Atomic force microscopy (AFM) experiments and molecular dynamics simulations were applied to determine the persistence length of individual infernan chains before studying their association induced by calcium. Infernan-based microgels were then produced using microfluidics and their mechanical properties were characterized by AFM methods. The mechanical properties of EPS/calcium microgels were finely tuned by varying the crosslinking density of their network, either by calcium or EPS concentrations. The obtained set of viscoelastic microgels with different elastic modulus values opens several possibilities for their applications in tissue engineering. 
PY 2022
PD SEP
SO Carbohydrate Polymers
SN 0144-8617
PU Elsevier BV
VL 292
UT 000806363700002
DI 10.1016/j.carbpol.2022.119629
ID 88367
ER

EF