Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis

Type Article
Date 2022-06
Language English
Author(s) Darius Hélène TaianaORCID1, Revel Taina1, Viallon Jérôme1, Sibat ManoellaORCID2, Cruchet Philippe1, Longo Sébastien1, Hardison Donnie Ransom3, Holland William C.3, Tester Patricia A.4, Litaker R. WayneORCID5, McCall Jennifer R.6, Hess PhilippORCID2, Chinain MireilleORCID1
Affiliation(s) 1 : Institut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French Polynesia
2 : IFREMER, PHYTOX, Laboratoire METALG, F-44000 Nantes, France
3 : National Oceanic and Atmospheric Administration, Center for Coastal Fisheries and Habitat Research, Beaufort, NC 28516, USA
4 : Ocean Tester, LLC, Beaufort, NC 28516, USA
5 : CSS, Inc. Under Contract to National Oceanic and Atmospheric Administration, National Centers for Coastal Ocean Science, National Ocean Service, Beaufort, NC 28516, USA
6 : Center for Marine Science, University of North Carolina Wilmington, 601 South College Road, Wilmington, NC 28403, USA
Source Marine Drugs (1660-3397) (MDPI AG), 2022-06 , Vol. 20 , N. 6 , P. 348 (30p.)
DOI 10.3390/md20060348
WOS© Times Cited 1
Note This article belongs to the Special Issue Development and Application of Specific Binding Assays for Marine Toxins
Keyword(s) ciguatera poisoning, Gambierdiscus polynesiensis, ciguatoxins, fluorescent receptor binding assay, neuroblastoma cell-based assay, liquid chromatography tandem mass spectrometry, risk assessment

Gambierdiscus and Fukuyoa dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of Gambierdiscus polynesiensis. fRBA was applied to Gambierdiscus matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (r2 = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in Gambierdiscus. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management.

Full Text
File Pages Size Access
Publisher's official version 30 3 MB Open access
Supplementary File 1 107 bytes Open access
Top of the page

How to cite 

Darius Hélène Taiana, Revel Taina, Viallon Jérôme, Sibat Manoella, Cruchet Philippe, Longo Sébastien, Hardison Donnie Ransom, Holland William C., Tester Patricia A., Litaker R. Wayne, McCall Jennifer R., Hess Philipp, Chinain Mireille (2022). Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis. Marine Drugs, 20(6), 348 (30p.). Publisher's official version : , Open Access version :