FN Archimer Export Format
PT J
TI Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis
BT 
AF Darius, Hélène Taiana
   Revel, Taina
   Viallon, Jérôme
   Sibat, Manoella
   Cruchet, Philippe
   Longo, Sébastien
   Hardison, Donnie Ransom
   Holland, William C.
   Tester, Patricia A.
   Litaker, R. Wayne
   McCall, Jennifer R.
   Hess, Philipp
   Chinain, Mireille
AS 1:1;2:1;3:1;4:2;5:1;6:1;7:3;8:3;9:4;10:5;11:6;12:2;13:1;
FF 1:;2:;3:;4:PDG-ODE-PHYTOX-METALG;5:;6:;7:;8:;9:;10:;11:;12:PDG-ODE-PHYTOX;13:;
C1 Institut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French Polynesia
   IFREMER, PHYTOX, Laboratoire METALG, F-44000 Nantes, France
   National Oceanic and Atmospheric Administration, Center for Coastal Fisheries and Habitat Research, Beaufort, NC 28516, USA
   Ocean Tester, LLC, Beaufort, NC 28516, USA
   CSS, Inc. Under Contract to National Oceanic and Atmospheric Administration, National Centers for Coastal Ocean Science, National Ocean Service, Beaufort, NC 28516, USA
   Center for Marine Science, University of North Carolina Wilmington, 601 South College Road, Wilmington, NC 28403, USA
C2 INSTITUT LOUIS MALARDE (ILM), FRANCE
   IFREMER, FRANCE
   NOAA, USA
   OCEAN TESTER LLC, USA
   NOAA, USA
   UNIV NORTH CAROLINA WILMINGTON, USA
SI NANTES
SE PDG-ODE-PHYTOX-METALG
   PDG-ODE-PHYTOX
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-int-hors-europe
IF 5.4
TC 10
UR https://archimer.ifremer.fr/doc/00773/88520/94234.pdf
   https://archimer.ifremer.fr/doc/00773/88520/94235.zip
LA English
DT Article
DE ;ciguatera poisoning;Gambierdiscus polynesiensis;ciguatoxins;fluorescent receptor binding assay;neuroblastoma cell-based assay;liquid chromatography tandem mass spectrometry;risk assessment
AB Gambierdiscus and Fukuyoa dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of Gambierdiscus polynesiensis. fRBA was applied to Gambierdiscus matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (r2 = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in Gambierdiscus. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management. 
PY 2022
PD JUL
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 20
IS 6
UT 000819605500001
DI 10.3390/md20060348
ID 88520
ER

EF