TY - JOUR T1 - Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass A1 - Vagner,Marie A1 - Boudry,G. A1 - Courcot,L. A1 - Vincent,D. A1 - Dehaut,A. A1 - Duflos,G. A1 - Huvet,Arnaud A1 - Tallec,K A1 - Zambonino-Infante,Jose-Luis AD - Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France AD - Institut Numecan, INRAE, INSERM, Univ Rennes, F-35590 Saint-Gilles, France AD - Laboratoire d’Océanologie et de Géosciences, Université Littoral Côte d’Opale, University of Lille, CNRS, UMR 8187, LOG, 32 avenue Foch, F-62930 Wimereux, France AD - Office Français de la Biodiversité (OFB), Direction Surveillance Évaluation et Données (DSUED), Service Écosystèmes Connaissances et Usages des milieux marins (ECUMM), 16 quai de la Douane, F-29200 Brest, France AD - ANSES Laboratoire de Sécurité des Aliments, 6 Boulevard du Bassin Napoléon, F-62200 Boulogne-sur-Mer, France AD - Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France UR - https://archimer.ifremer.fr/doc/00776/88753/ DO - 10.1016/j.envint.2022.107340 KW - Polystyrene nanoplastic beads KW - Translocation KW - Intestinal epithelium KW - Dicentrarchus labrax KW - Ussing chambers N2 - Plastic pollution in marine ecosystems constitutes an important threat to marine life. For vertebrates, macro/microplastics can obstruct and/or transit into the airways and digestive tract whereas nanoplastics (NPs; < 1000 nm) have been observed in non-digestive tissues such as the liver and brain. Whether NPs cross the intestinal epithelium to gain access to the blood and internal organs remains controversial, however. Here, we show directly NP translocation across the intestinal barrier of a fish, the European seabass, Dicentrarchus labrax, ex vivo. The luminal side of median and distal segments of intestine were exposed to fluorescent polystyrene NPs (PS-NPs) of 50 nm diameter. PS-NPs that translocated to the serosal side were then detected quantitatively by fluorimetry, and qualitatively by scanning electron microscopy (SEM) and pyrolysis coupled to gas chromatography and high-resolution mass spectrometry (Py-GC-HRMS). Fluorescence intensity on the serosal side increased 15–90 min after PS-NP addition into the luminal side, suggesting that PS-NPs crossed the intestinal barrier; this was confirmed by both SEM and Py-GC-HRMS. This study thus evidenced conclusively that NPs beads translocate across the intestinal epithelium in this marine vertebrate. Y1 - 2022/08 PB - Elsevier BV JF - Environment International SN - 0160-4120 VL - 166 ID - 88753 ER -