FN Archimer Export Format
PT J
TI Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass
BT 
AF Vagner, Marie
   Boudry, G.
   Courcot, L.
   Vincent, D.
   Dehaut, A.
   Duflos, G.
   Huvet, Arnaud
   Tallec, K
   Zambonino-Infante, Jose-Luis
AS 1:6;2:2;3:3;4:4;5:5;6:5;7:1;8:1;9:1;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-PFOM-LPI;8:;9:PDG-RBE-PFOM;
C1 Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
   Institut Numecan, INRAE, INSERM, Univ Rennes, F-35590 Saint-Gilles, France
   Laboratoire d’Océanologie et de Géosciences, Université Littoral Côte d’Opale, University of Lille, CNRS, UMR 8187, LOG, 32 avenue Foch, F-62930 Wimereux, France
   Office Français de la Biodiversité (OFB), Direction Surveillance Évaluation et Données (DSUED), Service Écosystèmes Connaissances et Usages des milieux marins (ECUMM), 16 quai de la Douane, F-29200 Brest, France
   ANSES Laboratoire de Sécurité des Aliments, 6 Boulevard du Bassin Napoléon, F-62200 Boulogne-sur-Mer, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
C2 IFREMER, FRANCE
   UNIV RENNES, FRANCE
   UNIV LITTORAL COTE D'OPALE, FRANCE
   OFB, FRANCE
   ANSES, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-RBE-PFOM-LPI
   PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 11.8
TC 19
UR https://archimer.ifremer.fr/doc/00776/88753/94427.pdf
   https://archimer.ifremer.fr/doc/00776/88753/94538.docx
LA English
DT Article
DE ;Polystyrene nanoplastic beads;Translocation;Intestinal epithelium;Dicentrarchus labrax;Ussing chambers
AB Plastic pollution in marine ecosystems constitutes an important threat to marine life. For vertebrates, macro/microplastics can obstruct and/or transit into the airways and digestive tract whereas nanoplastics (NPs; < 1000 nm) have been observed in non-digestive tissues such as the liver and brain. Whether NPs cross the intestinal epithelium to gain access to the blood and internal organs remains controversial, however. Here, we show directly NP translocation across the intestinal barrier of a fish, the European seabass, Dicentrarchus labrax, ex vivo. The luminal side of median and distal segments of intestine were exposed to fluorescent polystyrene NPs (PS-NPs) of 50 nm diameter. PS-NPs that translocated to the serosal side were then detected quantitatively by fluorimetry, and qualitatively by scanning electron microscopy (SEM) and pyrolysis coupled to gas chromatography and high-resolution mass spectrometry (Py-GC-HRMS). Fluorescence intensity on the serosal side increased 15–90 min after PS-NP addition into the luminal side, suggesting that PS-NPs crossed the intestinal barrier; this was confirmed by both SEM and Py-GC-HRMS. This study thus evidenced conclusively that NPs beads translocate across the intestinal epithelium in this marine vertebrate. 
PY 2022
PD AUG
SO Environment International
SN 0160-4120
PU Elsevier BV
VL 166
UT 000818498400001
DI 10.1016/j.envint.2022.107340
ID 88753
ER

EF