FN Archimer Export Format
PT J
TI High Throughput Screening of Antimicrobial Resistance Genes in Gram-Negative Seafood Bacteria
BT 
AF Delannoy, Sabine
   Hoffer, Corine
   Youf, Raphaëlle
   Dauvergne, Emilie
   Webb, Hattie E.
   Brauge, Thomas
   Tran, Mai-Lan
   Midelet, Graziella
   Granier, Sophie A.
   Haenni, Marisa
   Fach, Patrick
   Brisabois, Anne
AS 1:1;2:1;3:1;4:1;5:2;6:3;7:1;8:3;9:4;10:5;11:1;12:6;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 COLiPATH Unit & Genomics Platform IdentyPath, Laboratory for Food Safety, ANSES, 94700 Maisons-Alfort, France
   Department of Animal and Food Sciences, International Center for Food Safety Excellence, Texas Tech University, Lubbock, TX 79409, USA
   Bacteriology and Parasitology of Fishery and Aquaculture Products Unit, Laboratory for Food Safety, ANSES, 62200 Boulogne-sur-Mer, France
   Antibiotics, Biocides, Residues and Resistance Unit, Fougères Laboratory, ANSES, 35306 Fougères, France
   Antimicrobial Resistance and Bacterial Virulence Unit, Lyon Laboratory, Université de Lyon, ANSES, 69364 Lyon, France
   Strategy and Programs Department, ANSES, 94700 Maisons-Alfort, France
C2 ANSES, FRANCE
   UNIV TEXAS, USA
   ANSES, FRANCE
   ANSES, FRANCE
   ANSES, FRANCE
   ANSES, FRANCE
IN DOAJ
IF 4.5
TC 8
UR https://archimer.ifremer.fr/doc/00777/88912/94522.pdf
   https://archimer.ifremer.fr/doc/00777/88912/94523.zip
LA English
DT Article
CR CAMANOC
BO Thalassa
DE ;antimicrobial resistance genes;mobile genetic elements;seafood bacteria;Gram-negative species;high throughput qPCR;micro-array
AB From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regarding the content of resistance markers in various seafood samples and sources, and therefore the potential exposure to humans in a global One Health approach. An innovative high throughput qPCR screening was developed and validated in order to simultaneously investigate the presence of 41 ARGs and 33 MGEs including plasmid replicons, integrons, and insertion sequences in Gram-negative bacteria. Analysis of 268 seafood isolates from the bacterial microflora of cod (n = 24), shellfish (n = 66), flat fishes (n = 53), shrimp (n = 10), and horse mackerel (n = 115) show the occurrence of sul-1, ant(3″)-Ia, aph(3′)-Ia, strA, strB, dfrA1, qnrA, and blaCTX-M-9 genes in Pseudomonas spp., Providencia spp., Klebsiella spp., Proteus spp., and Shewanella spp. isolates and the presence of MGEs in all bacterial species investigated. We found that the occurrence of MGE may be associated with the seafood type and the environmental, farming, and harvest conditions. Moreover, even if MGE were detected in half of the seafood isolates investigated, association with ARG was only identified for twelve isolates. The results corroborate the hypothesis that the incidence of antimicrobial-resistant bacteria (ARB) and ARG decreases with increasing distance from potential sources of fecal contamination. This unique and original high throughput micro-array designed for the screening of ARG and MGE in Gram-negative bacteria could be easily implementable for monitoring antimicrobial resistance gene markers in diverse contexts. 
PY 2022
PD JUL
SO Microorganisms
SN 2076-2607
PU MDPI AG
VL 10
IS 6
UT 000815871400001
DI 10.3390/microorganisms10061225
ID 88912
ER

EF