FN Archimer Export Format
PT J
TI Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme
BT 
AF LOPEZ, Pierre
   Bridel, Sébastien
   SAULNIER, Denis
   David, Rarahu
   Magariños, Beatriz
   Torres, Beatriz S.
   Bernardet, Jean François
   Duchaud, Eric
AS 1:1,2;2:2;3:1;4:3;5:4;6:4;7:2;8:2;
FF 1:PDG-RBE-RMPF;2:;3:PDG-RBE-RMPF;4:;5:;6:;7:;8:;
C1 Ifremer, IRD Institut Louis‐ Malardé Univ Polynésie française EIO Labex Corail F‐ 98719 Taravao, Tahiti, Polynésie française, France
   Université Paris‐Saclay, INRAE UVSQ VIM Jouy‐en‐Josas 78350, France
   DRM ,Direction des Ressources Marines Fare Ute Immeuble Le caill Papeete, Tahiti, F‐98713 ,French Polynesia
   Departamento de Microbiología Facultad de Biología / CIBUS, Universidad de Santiago de Compostela Santiago de Compostela 15782 ,Spain
C2 IFREMER, FRANCE
   UNIV PARIS SACLAY, FRANCE
   DIRECT RESSOURCES MARINES, FRANCE
   UNIV SANTIAGO DE COMPOSTELA, SPAIN
SI TAHITI
SE PDG-RBE-RMPF
UM EIO
IN WOS Ifremer UMR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 4.3
TC 6
UR https://archimer.ifremer.fr/doc/00779/89073/94629.pdf
   https://archimer.ifremer.fr/doc/00779/89073/94630.pdf
   https://archimer.ifremer.fr/doc/00779/89073/94631.pdf
   https://archimer.ifremer.fr/doc/00779/89073/94632.xlsx
   https://archimer.ifremer.fr/doc/00779/89073/94633.xlsx
LA English
DT Article
DE ;aquaculture;fish diseases;molecular serotyping;o-antigen gene cluster;tenacibaculosis;Tenacibaculum maritimum
AB Tenacibaculum maritimum is a devastating bacterial pathogen affecting a large variety of marine fish species. It is responsible for significant economic losses in aquaculture farms worldwide. Different typing methods have been proposed to analyze bacterial diversity and population structure. Serological heterogeneity has been observed and up to four different serotypes have been described so far. However, the underlying molecular factors remain unknown. By combining conventional serotyping and genome-wide association study, we identified the genomic loci likely involved in the O-antigen biosynthesis. This finding allowed the development of a robust multiplex PCR-based serotyping scheme able to detect subgroups within each serotype and therefore performs better than conventional serotyping. This scheme was successfully applied to a large number of isolates from worldwide origin and retrieved from a large variety of fish species. No obvious correlations were observed between the mPCR-based serotype and the host species or the geographic origin of the isolates. Strikingly, the distribution of mPCR-based serotypes does not follow the core-genome phylogeny. Nevertheless, this simple and cost-effective mPCR-based serotyping method could be useful for different applications such as population structure analysis, disease surveillance, vaccine formulation and efficacy follow-up. 
PY 2022
PD SEP
SO Transboundary And Emerging Diseases
SN 1865-1674
PU Wiley
VL 69
IS 5
UT 000822020000001
DI 10.1111/tbed.14637
ID 89073
ER

EF