FN Archimer Export Format
PT J
TI A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment
BT 
AF Bernard, Clément
   Locard-Paulet, Marie
   Noël, Cyril
   Duchateau, Magalie
   Giai Gianetto, Quentin
   Moumen, Bouziane
   Rattei, Thomas
   Hechard, Yann
   Jensen, Lars Juhl
   Matondo, Mariette
   Samba-Louaka, Ascel
AS 1:1;2:2;3:3;4:4;5:4,5;6:1;7:6;8:1;9:2;10:4;11:1;
FF 1:;2:;3:PDG-IRSI-SEBIMER;4:;5:;6:;7:;8:;9:;10:;11:;
C1 Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS, 7267, Poitiers, France
   Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
   IFREMER-IRSI-Service de Bioinformatique (SeBiMER), Centre Bretagne, Plouzane, France
   Institut Pasteur, Université de Paris, Proteomics Platform, Mass Spectrometry for Biology Unit, UAR2024, CNRS 2000, Paris, France
   Institut Pasteur, Université de Paris, Department of Computation Biology, Bioinformatics and Biostatistics Hub, Paris, France
   Centre for Microbiology and Environmental Systems Science; Doctoral School Microbiology and Environmental Science, University of Vienna, Vienna, Austria
C2 UNIV POITIERS, FRANCE
   UNIV COPENHAGEN, DENMARK
   IFREMER, FRANCE
   INST PASTEUR, FRANCE
   INST PASTEUR, FRANCE
   UNIV VIENNA, AUSTRIA
SI BREST
SE PDG-IRSI-SEBIMER
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 16.6
TC 12
UR https://archimer.ifremer.fr/doc/00785/89743/95237.pdf
   https://archimer.ifremer.fr/doc/00785/89743/95238.pdf
   https://archimer.ifremer.fr/doc/00785/89743/95239.pdf
   https://archimer.ifremer.fr/doc/00785/89743/95240.docx
   https://archimer.ifremer.fr/doc/00785/89743/95241.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95242.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95243.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95244.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95245.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95246.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95247.xlsx
   https://archimer.ifremer.fr/doc/00785/89743/95248.pdf
   https://archimer.ifremer.fr/doc/00785/89743/95249.xlsx
LA English
DT Article
AB Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae. 
PY 2022
PD JUN
SO Nature Communications
SN 2041-1723
PU Springer Science and Business Media LLC
VL 13
IS 1
UT 000825733500004
DI 10.1038/s41467-022-31832-0
ID 89743
ER

EF