FN Archimer Export Format PT J TI De Novo Transcriptome Assembly and Analysis of the Flat Oyster Pathogenic Protozoa Bonamia Ostreae BT AF Chevignon, Germain Dotto-Maurel, Aurelie Serpin, Delphine Chollet, Bruno Arzul, Isabelle AS 1:1;2:1;3:1;4:1;5:1; FF 1:PDG-RBE-ASIM;2:PDG-RBE-ASIM;3:PDG-RBE-ASIM;4:PDG-RBE-ASIM;5:PDG-RBE-ASIM; C1 Ifremer, ASIM, La Tremblade, France C2 IFREMER, FRANCE SI LA TREMBLADE SE PDG-RBE-ASIM IN WOS Ifremer UPR DOAJ IF 5.7 TC 2 UR https://archimer.ifremer.fr/doc/00787/89874/95350.pdf LA English DT Article DE ;Bonamia ostreae;protozoan parasite;Haplosporida;RNAseq;Ostrea edulis;flat oyster;oyster AB The flat oyster Ostrea edulis is an oyster species native to Europe. It has declined to functional extinction in many areas of the NE Atlantic for several decades. Factors explaining this decline include over-exploitation of natural populations and diseases like bonamiosis, regulated across both the EU and the wider world and caused by the intracellular protozoan parasite Bonamia ostreae. To date, very limited sequence data are available for this Haplosporidian species. We present here the first transcriptome of B. ostreae. As this protozoan is not yet culturable, it remains extremely challenging to obtain high-quality -omic data. Thanks to a specific parasite isolation protocol and a dedicated bioinformatic pipeline, we were able to obtain a high-quality transcriptome for an intracellular marine micro-eukaryote, which will be very helpful to better understand its biology and to consider the development of new relevant diagnostic tools. PY 2022 PD JUN SO Frontiers In Cellular And Infection Microbiology SN 2235-2988 PU Frontiers Media SA VL 12 UT 000834004900001 DI 10.3389/fcimb.2022.921136 ID 89874 ER EF